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Physicochemical properties and acute toxicity studies of a lectin from the saline extract of the fruiting bodies of the shiitake mushroom, Lentinula edodes (Berk).
MedLine Citation:
PMID:  22187665     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A lectin (LEL) was isolated from the fresh fruiting bodies of the shiitake mushroom Lentinula edodes by a combination of gel filtration chromatography on Sephadex G-150 and affinity chromatography on an N-acetyl-Dgalactosamine-Sepharose 4B column. Its molecular mass, as determined by gel filtration, was estimated to be 71, 000 Daltons and its structure is homotetrameric with subunit molecular weight of approximately 18,000 Daltons. LEL agglutinated non-specifically red blood cells from the human ABO system as well as rabbit erythrocytes and in haemagglutination inhibition assays, exhibited sugar-binding specificity toward N-acetyl-D-galactosamine. EDTA had no inhibitory effect on its haemagglutinating activity, which was stable up to 70°C and was not affected by changes in pH. The lectin had no covalently-linked carbohydrate and amino acid composition analysis revealed that it contained 124 amino acid residues and was rich in tyrosine, proline, phenylalanine, arginine, glutamic acid and cysteine. LEL did not cause mortality neither was it observed to alter the morphology of key organs when administered intraperitoneally at concentrations up to 10,000 mg kg(-1) body weight of mice.
Authors:
Queensley Eghianruwa; Oludele Odekanyin; Adenike Kuku
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Publication Detail:
Type:  Journal Article     Date:  2011-11-10
Journal Detail:
Title:  International journal of biochemistry and molecular biology     Volume:  2     ISSN:  2152-4114     ISO Abbreviation:  Int J Biochem Mol Biol     Publication Date:  2011  
Date Detail:
Created Date:  2011-12-21     Completed Date:  2012-10-02     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101532076     Medline TA:  Int J Biochem Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  309-17     Citation Subset:  -    
Affiliation:
Department of Biochemistry, Obafemi Awolowo University Ile-Ife Nigeria.
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