| Physicochemical and biological characterization of targeted, nucleic acid-containing nanoparticles. | |
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MedLine Citation:
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PMID: 17326672 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nucleic acid-based therapeutics have the potential to provide potent and highly specific treatments for a variety of human ailments. However, systemic delivery continues to be a significant hurdle to success. Multifunctional nanoparticles are being investigated as systemic, nonviral delivery systems, and here, we describe the physicochemical and biological characterization of cyclodextrin-containing polycations (CDP) and their nanoparticles formed with nucleic acids including plasmid DNA (pDNA) and small interfering RNA (siRNA). These polycation/nucleic acid complexes can be tuned by formulation conditions to yield particles with sizes ranging from 60 to 150 nm, zeta potentials from 10 to 30 mV, and molecular weights from approximately 7 x 107 to 1 x 109 g mol-1 as determined by light scattering techniques. Inclusion complexes formed between adamantane (AD)-containing molecules and the beta-cyclodextrin molecules enable the modular attachment of poly(ethylene glycol) (AD-PEG) conjugates for steric stabilization and targeting ligands (AD-PEG-transferrin) for cell-specific targeting. A 70 nm particle can contain approximately 10 000 CDP polymer chains, approximately 2000 siRNA molecules, approximately 4000 AD-PEG5000 molecules, and approximately 100 AD-PEG5000-Tf molecules; this represents a significant payload of siRNA and a large ratio of siRNA to targeting ligand (20:1). The particles protect the nucleic acid payload from nuclease degradation, do not aggregate at physiological salt concentrations, and cause minimal erythrocyte aggregation and complement fixation at the concentrations typically used for in vivo application. Uptake of the nucleic acid-containing particles by HeLa cells is measured by flow cytometry and visualized by confocal microscopy. Competitive uptake experiments show that the transferrin-targeted particles display enhanced affinity for the transferrin receptor through avidity effects (multiligand binding). Functional efficacy of the delivered pDNA and siRNA is demonstrated through luciferase reporter protein expression and knockdown, respectively. The analysis of the CDP delivery vehicle provides insights that can be applied to the design of targeted nucleic acid delivery vehicles in general. |
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Authors:
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Derek W Bartlett; Mark E Davis |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-02-28 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 18 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2007 Mar-Apr |
Date Detail:
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Created Date: 2007-03-21 Completed Date: 2007-05-03 Revised Date: 2012-01-20 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 456-68 Citation Subset: IM |
Affiliation:
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Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adamantane
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metabolism Animals Cattle Cyclodextrins / chemistry*, metabolism Drug Carriers / chemistry*, metabolism Drug Delivery Systems Electrophoretic Mobility Shift Assay Erythrocyte Aggregation Gene Transfer Techniques HeLa Cells Humans Nanoparticles* Nucleic Acids / chemistry*, metabolism Plasmids Polyamines / chemistry*, metabolism Polyethylene Glycols / chemistry RNA, Small Interfering Receptors, Transferrin / metabolism Transferrin / chemistry*, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1 R01 EB004657-01/EB/NIBIB NIH HHS; R01 EB004657-01/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cyclodextrins; 0/Drug Carriers; 0/Nucleic Acids; 0/Polyamines; 0/Polyethylene Glycols; 0/RNA, Small Interfering; 0/Receptors, Transferrin; 0/Transferrin; 0/polycations; 281-23-2/Adamantane |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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