Document Detail


Physicochemical Properties and Antimicrobial Efficacy of Electrostatic Complexes based on Cationic ε-Polylysine and Anionic Pectin.
MedLine Citation:
PMID:  21574554     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ε-Polylysine (ε-PL) is a food-grade cationic antimicrobial that is highly effective against a wide range of food pathogens and spoilage organisms. However, its application within foods and beverages is currently limited because of its tendency to associate with anionic substances, thereby increasing product turbidity or forming sediments. In this study, we examined a potential means of overcoming these problems by forming electrostatic complexes between cationic ε-PL and anionic pectin. The nature of the complexes formed depended on the mass ratio of pectin-to-ε-PL (RP-PL), since this determined their electrical characteristics, aggregation stability, and antimicrobial efficacy. The electrical charge on the complexes went from positive to negative with increasing RP-PL, with the point of zero charge being around RP-PL ~ 8. Soluble complexes or stable colloidal dispersions were formed at low and high RP-PL levels, but insoluble complexes were formed at intermediate levels (i.e. 4 < RP-PL < 20). The complexes maintained good antimicrobial activity (minimum inhibitory concentration of ε-PL < 10 μg/ml) at RP-PL ≤ 20 against two acid resistant spoilage yeasts: Zygosaccharomyces bailli and Saccharomyces cerevisiae. Finally, we showed that certain ε-PL-pectin complexes (10 μg/ml ε-PL; RP-PL ≥ 2) could be incorporated into green tea beverages without adversely affecting their appearance or physical stability. This work has shown that the function of a cationic antimicrobial agent (ε-polylysine) can be improved by incorporating it within electrostatic complexes using a food-grade anionic biopolymer (pectin).
Authors:
Yuhua Chang; Lynne McLandsborough; David Julian McClements
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-16
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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