Document Detail

Physico-chemical characterization and biological evaluation of two fibroin materials.
MedLine Citation:
PMID:  22972624     Owner:  NLM     Status:  Publisher    
Silk fibroin fibres from two different sources, Bombyx mori pure-breed silkworms and polyhybrid cross-bred silkworm cocoons, were treated with formic acid under planar stirring conditions to prepare non-woven nets. The treatment partially dissolved the fibres, which bound together and formed a non-woven micrometric net with fibres coated by a thin layer of low molecular weight fibroin matrix. The starting fibres, net materials and fibroin coating layer were characterized in terms of amino acid composition, molecular weight and calorimetric properties. In vitro cell culture tests with rat fibroblasts were performed to investigate cell proliferation, morphology and spreading. Moreover, host-rat fibroblasts were preseeded on the afore-mentioned nets and implanted in the thorax of rats for histological analysis. In spite of the chemical differences between the two starting fibroins, the response of the said materials in vitro and in vivo were very similar. These results suggest that the outcome is likely correlated with the modification of the processing technique; that during the formation of the net, a thin gel layer of similar amino acid composition was formed on the fibroin fibres. Copyright © 2012 John Wiley & Sons, Ltd.
Antonella Motta; Paola Segnana; Lucia Verin; Silvia La Monica; Claudia Fumarola; Giovanna Bucci; Francesca Gussago; Anna Maria Cantoni; Luca Ampollini; Claudio Migliaresi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-13
Journal Detail:
Title:  Journal of tissue engineering and regenerative medicine     Volume:  -     ISSN:  1932-7005     ISO Abbreviation:  J Tissue Eng Regen Med     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101308490     Medline TA:  J Tissue Eng Regen Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Department of Materials Engineering and Industrial Technologies and Biotech Research Centre, University of Trento, Italy; European Institute of Excellence on Tissue Engineering and Regenerative Medicine, Trento, Italy.
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