Document Detail


Physical stability of different liposome compositions obtained by extrusion method.
MedLine Citation:
PMID:  8544096     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Six different liposome compositions were evaluated according their physical stability. The compositions used were (in mole ratio): dipalmitoylphosphatidylcholine (DPPC): DPPC:cholesterol (CHOL) (1:1), DPPC:CHOL (1:6:1), DPPC:CHOL:sphingomyelin (SFM) (7:2:1), DPPC:CHOL:stearylamine (STE) (8:5:1) and DPPC:CHOL:dicetyl phosphate (DCP) (8:5:1). The liposomes were obtained by the extrusion method, through polycarbonate membranes of 0.1 micron pore size. The captured volume, number of lamellae, size and polydispersity of the vesicle populations were determined for all compositions. The physical stability was checked at -20 degrees C, room temperature, 4 degrees C and 50 degrees C by determining the changes in vesicle size over a maximum of 40 days. The process of aggregation and/or fusion was observed by photon correlation spectroscopy. From the results, we can establish that the above compositions are metastable at a temperature of 50 degrees C. On the other hand, values of captured volume were smaller than predicted by theory. This fact can be explained by the non-sphericity of extruded vesicles. In relation to the stability, the introduction of CHOL in the formulation allows keeping the vesicles at 4 degrees C. In contrast, liposomes containing only DPPC are very stable at room temperature. Compositions with a high stability are those that have present SFM or STE. The latter keeps the structural bilayer at temperatures < 0 degrees C without cryoprotectors. Both the lipids, STE and DCP, form vesicles with a higher number of lamellae.
Authors:
X Armengol; J Estelrich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microencapsulation     Volume:  12     ISSN:  0265-2048     ISO Abbreviation:  J Microencapsul     Publication Date:    1995 Sep-Oct
Date Detail:
Created Date:  1996-02-13     Completed Date:  1996-02-13     Revised Date:  2009-07-21    
Medline Journal Info:
Nlm Unique ID:  8500513     Medline TA:  J Microencapsul     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  525-35     Citation Subset:  IM    
Affiliation:
Unitat de Fisicoquímica, Facultat de Farmàcia, Universitat de Barcelona, Catalonia, Spain.
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MeSH Terms
Descriptor/Qualifier:
Absorptiometry, Photon
Drug Stability
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Lipids / chemistry
Liposomes / chemistry*
Particle Size
Temperature
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Lipids; 0/Liposomes; 3301-79-9/6-carboxyfluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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