Document Detail


Physical monitoring of mating type switching in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  2841579     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The kinetics of mating type switching in Saccharomyces cerevisiae can be followed at the DNA level by using a galactose-inducible HO (GAL-HO) gene to initiate the event in synchronously growing cells. From the time that HO endonuclease cleaves MAT a until the detection of MAT alpha DNA took 60 min. When unbudded G1-phase cells were induced, switched to the opposite mating type in "pairs." In the presence of the DNA synthesis inhibitor hydroxyurea, HO-induced cleavage occurred but cells failed to complete switching. In these blocked cells, the HO-cut ends of MATa remained stable for at least 3 h. Upon removal of hydroxyurea, the cells completed the switch in approximately 1 h. The same kinetics of MAT switching were also seen in asynchronous cultures and when synchronously growing cells were induced at different times of the cell cycle. Thus, the only restriction that confined normal homothallic switching to the G1 phase of the cell cycle was the expression of HO endonuclease. Further evidence that galactose-induced cells can switch in the G2 phase of the cell cycle was the observation that these cells did not always switch in pairs. This suggests that two chromatids, both cleaved with HO endonuclease, can interact independently with the donors HML alpha and HMRa.
Authors:
B Connolly; C I White; J E Haber
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biology     Volume:  8     ISSN:  0270-7306     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-09-16     Completed Date:  1988-09-16     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2342-9     Citation Subset:  IM    
Affiliation:
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
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MeSH Terms
Descriptor/Qualifier:
DNA / metabolism
DNA Restriction Enzymes / metabolism
Electrophoresis, Agar Gel
Enzyme Induction
Galactose / pharmacology
Genes, Fungal*
Genes, Mating Type, Fungal*
Kinetics
Peptides / pharmacology*
Promoter Regions, Genetic
Saccharomyces cerevisiae / drug effects,  genetics*,  metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
GM20056/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Peptides; 26566-61-0/Galactose; 61194-02-3/mating factor; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

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