Document Detail


Physical mechanisms redirecting cell polarity and cell shape in fission yeast.
MedLine Citation:
PMID:  19026544     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cylindrical rod shape of the fission yeast Schizosaccharomyces pombe is organized and maintained by interactions between the microtubule, cell membrane, and actin cytoskeleton [1]. Mutations affecting any components in this pathway lead to bent, branched, or round cells [2]. In this context, the cytoskeleton controls cell polarity and thus dictates cell shape. Here, we use soft-lithography techniques to construct microfluidic channels to control cell shape. We show that when wild-type rod-shaped cells are physically forced to grow in a bent fashion, they will reorganize their cytoskeleton and redirect cell polarity to make new ectopic cell tips. Moreover, when bent or round mutant cells are physically forced to conform to the wild-type rod-shape, they will reverse their mutational phenotypes by reorganizing their cytoskeleton to maintain proper wild-type-like localization of microtubules, cell-membrane proteins, and actin. Our study provides direct evidence that the cytoskeleton controls cell polarity and cell shape and demonstrates that cell shape also controls the organization of the cytoskeleton in a feedback loop. We present a model of the feedback loop to explain how fission yeast maintain a rod shape and how perturbation of specific parameters of the loop can lead to different cell shapes.
Authors:
Courtney R Terenna; Tatyana Makushok; Guilhem Velve-Casquillas; Damien Baigl; Yong Chen; Michel Bornens; Anne Paoletti; Matthieu Piel; Phong T Tran
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current biology : CB     Volume:  18     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-11-27     Completed Date:  2009-01-27     Revised Date:  2013-06-04    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1748-53     Citation Subset:  IM    
Affiliation:
Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Enlargement
Cell Polarity / physiology*
Cell Shape*
Microfluidics
Microtubules / physiology*,  ultrastructure
Models, Biological
Morphogenesis
Mutation
Phenotype
Schizosaccharomyces / genetics,  growth & development,  ultrastructure*
Schizosaccharomyces pombe Proteins / genetics
Grant Support
ID/Acronym/Agency:
R01 GM070899-03/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Schizosaccharomyces pombe Proteins
Comments/Corrections
Comment In:
Curr Biol. 2009 Mar 10;19(5):R205-6   [PMID:  19278635 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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