Document Detail

Physical mapping of chromosome 8p22 markers and their homozygous deletion in a metastatic prostate cancer.
MedLine Citation:
PMID:  8661103     Owner:  NLM     Status:  MEDLINE    
Numerous studies have implicated the short arm of chromosome 8 as the site of one or more tumor suppressor genes inactivated in carcinogenesis of the prostate, colon, lung, and liver. Previously, we identified a homozygous deletion on chromosome 8p22 in a metastatic prostate cancer. To map this homozygous deletion physically, long-range restriction mapping was performed using yeast artificial chromosomes (YACs) spanning approximately 2 Mb of chromosome band 8p22. Subcloned genomic DNA and cDNA probes isolated by hybrid capture from these YACs were mapped in relation to one another, reinforcing map integrity. Mapped single-copy probes from the region were then applied to DNA isolated from a metastatic prostate cancer containing a chromosome 8p22 homozygous deletion and indicated that its deletion spans 730-970 kb. Candidate genes PRLTS (PDGF-receptor beta-like tumor suppressor) and CTSB (cathepsin B) are located outside the region of homozygous deletion. Généthon marker D8S549 is located approximately at the center of this region of homozygous deletion. Two new microsatellite polymorphisms, D8S1991 and D8S1992, also located within the region of homozygous deletion on chromosome 8p22, are described. Physical mapping places cosmid CI8-2644 telomeric to MSR (macrophage scavenger receptor), the reverse of a previously published map, altering the interpretation of published deletion studies. This work should prove helpful in the identification of candidate tumor suppressor genes in this region.
G S Bova; D MacGrogan; A Levy; S S Pin; R Bookstein; W B Isaacs
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  35     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-09-30     Completed Date:  1996-09-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  46-54     Citation Subset:  IM    
Department of Urology, The Johns Hopkins University School of Medicine and Brady Urological Institute, Baltimore, Maryland, 21287, USA.
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MeSH Terms
Adenocarcinoma / genetics,  secondary*
Base Sequence
Chromosomes, Artificial, Yeast / genetics
Chromosomes, Human, Pair 8 / genetics*
Cosmids / genetics
Lymphatic Metastasis / genetics*
Microsatellite Repeats
Middle Aged
Molecular Sequence Data
Prostatic Neoplasms / genetics*
Sequence Deletion*
Grant Support

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