| Physical and functional coupling of RNA-dependent RNA polymerase and Dicer in the biogenesis of endogenous siRNAs. | |
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MedLine Citation:
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PMID: 17603500 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many classes of small RNA (sRNA) involved in RNA silencing are generated by double-stranded RNA (dsRNA) processing. Although principles of sRNA biogenesis have emerged, newly identified classes of sRNAs have features that suggest additional biogenesis mechanisms. Tetrahymena thermophila expresses one such class, comprising sRNAs of 23 and 24 nucleotides (nt) with an absolute strand bias in accumulation. Here we demonstrate sRNA production by the T. thermophila Dicer Dcr2 and the RNA-dependent RNA polymerase Rdr1, which purifies as a multisubunit RNA-dependent RNA polymerase complex (RDRC). Dcr2 and RDRC interact, stimulating Dcr2 activity. Moreover, Dcr2 specificity is influenced by RDRC beyond this physical interaction, as Dcr2 generates discrete 23- and 24-nt sRNAs only from dsRNA with a 5'-triphosphate. These findings suggest that sRNA strand bias arises from Dcr2 processing polarity, conferred by physical and functional coupling of RDRC and Dicer enzymes. |
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Authors:
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Suzanne R Lee; Kathleen Collins |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-07-01 |
Journal Detail:
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Title: Nature structural & molecular biology Volume: 14 ISSN: 1545-9993 ISO Abbreviation: Nat. Struct. Mol. Biol. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-05 Completed Date: 2007-09-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101186374 Medline TA: Nat Struct Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 604-10 Citation Subset: IM |
Affiliation:
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Department of Molecular and Cell Biology, University of California - Berkeley, 142 Life Sciences Addition 3200, Berkeley, California 94720-3200, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Genes, Protozoan Molecular Sequence Data Protozoan Proteins / chemistry*, genetics, metabolism RNA Replicase / chemistry*, genetics, metabolism RNA, Protozoan / biosynthesis* RNA, Small Interfering / biosynthesis*, chemistry Ribonuclease III / chemistry*, metabolism Substrate Specificity Tetrahymena thermophila / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Protozoan Proteins; 0/RNA, Protozoan; 0/RNA, Small Interfering; EC 2.7.7.48/RNA Replicase; EC 3.1.26.3/Ribonuclease III |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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