Document Detail


Physical demonstration of a high molecular weight bacteriocin plasmid in Vibrio cholerae by genetic transformation process.
MedLine Citation:
PMID:  2345019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacteriocinogeny was transferred at high frequencies from bacteriocinogenic (Bac+) V. cholerae strains to non-bacteriocinogenic (Bac-) recipients in the in situ genetic transformation system on agar surface. DNA extracted from samples of growth of bacteria transformed to Bac+ were obtained at 2 h intervals following contact with the sterile agar surface where the donor had grown previously. This showed acquisition of a high molecular weight plasmid which could be physically demonstrated best in the 6 h sample of the Bac+ transformants; their 4 h samples failed to show this specific plasmid, while it was demonstrable only as a faint band in the 8 h samples.
Authors:
S Basu; A N Chakrabarty; M Ganguli; S G Dastidar
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Indian journal of medical research     Volume:  91     ISSN:  0971-5916     ISO Abbreviation:  Indian J. Med. Res.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-07-05     Completed Date:  1990-07-05     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0374701     Medline TA:  Indian J Med Res     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  120-3     Citation Subset:  IM    
Affiliation:
Department of Medical Microbiology & Parasitology, University College of Medicine, Calcutta University.
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MeSH Terms
Descriptor/Qualifier:
Bacteriocins / biosynthesis*
DNA, Bacterial / analysis*
Plasmids*
Transformation, Bacterial*
Vibrio cholerae / genetics*
Chemical
Reg. No./Substance:
0/Bacteriocins; 0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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