Document Detail

Physical and computational analysis of the yeast Kluyveromyces lactis secreted proteome.
MedLine Citation:
PMID:  18601269     Owner:  NLM     Status:  MEDLINE    
Secretion of proteins is the most common approach to protein expression in Kluyveromyces lactis. A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by K. lactis cells. Multidimensional separations were conducted and RP online ESI-MS/MS analysis identified 81 secreted proteins. In addition, an in silico analysis predicted 178 K. lactis proteins to be secreted via the general secretory pathway (GSP). These two datasets were compared and approximately 70% of the K. lactis proteins detected in the culture medium possessed a GSP sequence. The detected proteins included those involved with cell wall structure and synthesis, carbohydrate metabolism, and proteolysis, a result that may have significant bearing on heterologous protein expression. Additionally, both the experimental and in silico datasets were compared to similar, previously published datasets for Candida albicans. With the methodology presented here, we provide the deepest penetration into a yeast secretome yet reported.
Catherine L Swaim; Brian P Anton; Shamik S Sharma; Christopher H Taron; Jack S Benner
Related Documents :
3085689 - The major basic proteins of bull seminal vesicle secretion.
21899359 - Protein binding affinity of leucaena condensed tannins of differing molecular weights.
22386149 - Proclusensem: predicting membrane protein types by fusing different modes of pseudo ami...
313879 - Epidermal growth factor in the submandibular gland and serum of mice with muscular dyst...
22111639 - Differential protein expression in response to the phytoalexin brassinin allows the ide...
22065229 - Time-controlled transcardiac perfusion crosslinking for in vivo interactome studies.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proteomics     Volume:  8     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2008-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2714-23     Citation Subset:  IM    
New England Biolabs, Ipswich, MA 01938, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bioreactors / microbiology
Computational Biology / methods*
Computer Simulation
Culture Media / chemistry
Fungal Proteins / secretion*
Gene Expression Regulation, Fungal
Genes, Fungal
Kluyveromyces / genetics,  metabolism*
Models, Biological
Proteome / analysis*,  secretion
Proteomics / methods*
Reg. No./Substance:
0/Culture Media; 0/Fungal Proteins; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The role of thioredoxin TrxA in Bacillus subtilis: a proteomics and transcriptomics approach.
Next Document:  A novel membrane bioreactor for detoxifying industrial wastewater: I. Biodegradation of phenol in a ...