Document Detail


Physical and computational analysis of the yeast Kluyveromyces lactis secreted proteome.
MedLine Citation:
PMID:  18601269     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Secretion of proteins is the most common approach to protein expression in Kluyveromyces lactis. A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by K. lactis cells. Multidimensional separations were conducted and RP online ESI-MS/MS analysis identified 81 secreted proteins. In addition, an in silico analysis predicted 178 K. lactis proteins to be secreted via the general secretory pathway (GSP). These two datasets were compared and approximately 70% of the K. lactis proteins detected in the culture medium possessed a GSP sequence. The detected proteins included those involved with cell wall structure and synthesis, carbohydrate metabolism, and proteolysis, a result that may have significant bearing on heterologous protein expression. Additionally, both the experimental and in silico datasets were compared to similar, previously published datasets for Candida albicans. With the methodology presented here, we provide the deepest penetration into a yeast secretome yet reported.
Authors:
Catherine L Swaim; Brian P Anton; Shamik S Sharma; Christopher H Taron; Jack S Benner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proteomics     Volume:  8     ISSN:  1615-9861     ISO Abbreviation:  Proteomics     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-17     Completed Date:  2008-09-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101092707     Medline TA:  Proteomics     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2714-23     Citation Subset:  IM    
Affiliation:
New England Biolabs, Ipswich, MA 01938, USA.
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MeSH Terms
Descriptor/Qualifier:
Bioreactors / microbiology
Computational Biology / methods*
Computer Simulation
Culture Media / chemistry
Fermentation
Fungal Proteins / secretion*
Gene Expression Regulation, Fungal
Genes, Fungal
Kluyveromyces / genetics,  metabolism*
Models, Biological
Proteome / analysis*,  secretion
Proteomics / methods*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Fungal Proteins; 0/Proteome

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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