Document Detail

Physical and chemical properties of human plasma alpha2-macroglobulin.
MedLine Citation:
PMID:  80217     Owner:  NLM     Status:  MEDLINE    
Alpha2-M (alpha2-macroglobulin) was purified from human plasma by two different procedures. As well as having no detectable impurities by the usual criteria for testing the homogeneity of protein preparations, these alpha2M preparations showed a single component, after reduction in urea, of 185000 daltons by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The molecular weight of the alpha2M was found to be 718000 by sedimentation equilibrium experiments using the gravimetrically determined -v of 0.731 ml/g. The interaction of several proteinases with alpha2M was studied by using a novel discontinuous polyacrylamide-gel system, which showed clear separation of the enzyme-complexed alpha2M from the free alpha2M. These studies indicated that urokinase, as well as trypsin, chymotrypsin, plasmin and thrombin forms complexes with alphaM. The cleavage of the 185000-dalton subunit to a 85000-dalton species on interaction of trypsin with alpha2M was demonstrated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis after reduction of the alpha2M-trypsin complex in urea. The amino acid composition, carbohydrate content, absorption coefficient at 280 nm, the specific refractive increment and the sedimentation coefficient for these alpha2M preparations were measured. The stability of the trypsin-binding activity of the alpha2M preparations was also studied under several storage situations.
P K Hall; R C Roberts
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  173     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1978 Jul 
Date Detail:
Created Date:  1978-10-27     Completed Date:  1978-10-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  27-38     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Carbohydrates / analysis
Centrifugation, Density Gradient
Chemical Phenomena
Electrophoresis, Polyacrylamide Gel
Molecular Weight
alpha-Macroglobulins* / isolation & purification
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/alpha-Macroglobulins

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