Document Detail


Physical changes in specimens of five species of Cyprinidae preserved in ethanol and frozen.
MedLine Citation:
PMID:  25041177     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Preservation in 30% ethanol and freezing to a temperature of -20 ± 2° C is an appropriate method for measurement of fish eggs, larvae and juveniles. Egg diameter of the common carp Cyprinus carpio increased insignificantly by 1·32% after preservation compared with live size. The total length (LT ) of 1 day post-hatching (dph) larvae as well as the standard length (LS) of 16 dph larvae of C. carpio increased significantly (2·95 and 1·50%, respectively) after preservation. Egg diameter as well as the LT of 1 dph larvae of barbel Barbus barbus increased significantly after preservation, by 1·74 and 1·96%, respectively over their original size. The standard length (LS ) of 14 dph larvae of B. barbus as well as juveniles of B. barbus, crucian carp Carassius carassius, common nase Chondrostoma nasus and tench Tinca tinca decreased significantly after preservation (-0·56 to -5·54%), whereas their body mass increased significantly (11·46-18·57%). Preserved eggs of C. carpio and B. barbus were hard, round and transparent. The larvae and juveniles of examined fishes, preserved in frozen ethanol, were straight, flexible and easily measurable after 60 days. Integrity of body surface and fins, as well as preservation of colours were much better in larvae or juveniles frozen and thawed only once than in specimens frozen and thawed thrice. Cooling in 30% ethanol to a temperature of 6 ± 2° C and freezing in water to a temperature of -20 ± 2° C are not appropriate preservation methods for eggs and larvae of C. carpio (1 and 16 dph).
Authors:
M Korwin-Kossakowski
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-18
Journal Detail:
Title:  Journal of fish biology     Volume:  -     ISSN:  1095-8649     ISO Abbreviation:  J. Fish Biol.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0214055     Medline TA:  J Fish Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 The Fisheries Society of the British Isles.
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