Document Detail


Physical characterization and quantification of bacteria by sedimentation field-flow fractionation.
MedLine Citation:
PMID:  16348964     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Studies in microbial ecology require accurate measures of cell number and biomass. Although epifluorescence microscopy is an accepted and dependable method for determining cell numbers, current methods of converting biovolume to biomass are error prone, tedious, and labor-intensive. This paper describes a technique with sedimentation field-flow fractionation to enumerate bacteria and determine their density, size, and mass. Using cultured cells of different shapes and sizes, we determined optimum values for separation run parameters and sample-handling procedures. The technique described can separate and detect 4', 6-diamidino-2-phenylindole-stained cells and generate a fractogram from which cell numbers and their size or mass distribution can be calculated. A direct method for estimating bacterial biomass (dry organic matter content) which offers distinct advantages over present methods for calculating biomass has been developed.
Authors:
R V Sharma; R T Edwards; R Beckett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  59     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  2010-06-25     Completed Date:  2010-07-07     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1864-75     Citation Subset:  -    
Affiliation:
Water Studies Centre, Department of Chemistry, Monash University, 900 Dandenong Road, Caulfield East, Victoria, Australia 3145.
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