Document Detail


Phylogenetic relationships in three species of canine Demodex mite based on partial sequences of mitochondrial 16S rDNA.
MedLine Citation:
PMID:  23140317     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Background -  The historical classification of Demodex mites has been based on their hosts and morphological features. Genome sequencing has proved to be a very effective taxonomic tool in phylogenetic studies and has been applied in the classification of Demodex. Mitochondrial 16S rDNA has been demonstrated to be an especially useful marker to establish phylogenetic relationships. Hypothesis/Objectives -  To amplify and sequence a segment of the mitochondrial 16S rDNA from Demodex canis and Demodex injai, as well as from the short-bodied mite called, unofficially, D. cornei and to determine their genetic proximity. Methods -  Demodex mites were examined microscopically and classified as Demodex folliculorum (one sample), D. canis (four samples), D. injai (two samples) or the short-bodied species D. cornei (three samples). DNA was extracted, and a 338 bp fragment of the 16S rDNA was amplified and sequenced. Results -  The sequences of the four D. canis mites were identical and shared 99.6 and 97.3% identity with two D. canis sequences available at GenBank. The sequences of the D. cornei isolates were identical and showed 97.8, 98.2 and 99.6% identity with the D. canis isolates. The sequences of the two D. injai isolates were also identical and showed 76.6% identity with the D. canis sequence. Conclusion -  Demodex canis and D. injai are two different species, with a genetic distance of 23.3%. It would seem that the short-bodied Demodex mite D. cornei is a morphological variant of D. canis.
Authors:
Natalia Sastre; Ivan Ravera; Sergio Villanueva; Laura Altet; Mar Bardagí; Armand Sánchez; Olga Francino; Lluís Ferrer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Veterinary dermatology     Volume:  23     ISSN:  1365-3164     ISO Abbreviation:  Vet. Dermatol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426187     Medline TA:  Vet Dermatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  509-e101     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Veterinary Dermatology © 2012 ESVD and ACVD.
Affiliation:
Servei Veterinari de Genètica Molecular, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain Department of Animal Medicine and Surgery, Veterinary School, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain Department of Clinical Sciences, Cummings School of Veterinary Medicine, Tufts University, North Grafton, MA, USA.
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