| Phylogenetic relationship between human immunodeficiency virus type 1 (HIV-1) long terminal repeat natural variants present in the lymph node and peripheral blood of three HIV-1-infected individuals. | |
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MedLine Citation:
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PMID: 8021592 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PCR has been used to amplify a 540 base pair fragment of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat region encompassing the U5/R/U3 regions from proviral HIV DNA that was present in the lymph nodes and peripheral blood mononuclear cells of three HIV-infected individuals. The resulting PCR products were cloned and the DNA sequence of multiple clones from each reaction was determined to enable the distribution and phylogenetic relationship between the variants in each body site to be assessed. The results of bootstrapped parsimony phylogenetic analyses showed that a distinct polarization was evident between peripheral blood and lymph node variants in the patient who possessed a histologically intact lymph node. In contrast, similar analyses conducted on samples from two patients with extensive lymph node disruption showed similar variants in lymph node and peripheral blood. In the patient with an intact lymph node, lymph node variants were significantly more heterogeneous than those present in blood samples taken either simultaneously or 11 months later. No differences in heterogeneity between lymph node and peripheral blood were observed in patients with disrupted lymph nodes. The significance of these results for our understanding of HIV pathogenesis is discussed. |
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Authors:
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M Ait-Khaled; V C Emery |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of general virology Volume: 75 ( Pt 7) ISSN: 0022-1317 ISO Abbreviation: J. Gen. Virol. Publication Date: 1994 Jul |
Date Detail:
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Created Date: 1994-08-04 Completed Date: 1994-08-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0077340 Medline TA: J Gen Virol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1615-21 Citation Subset: IM; X |
Affiliation:
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Division of Communicable Diseases, Royal Free Hospital School of Medicine, Hampstead, London, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cloning, Molecular Consensus Sequence DNA, Viral / analysis, blood Genetic Variation / genetics* HIV Infections / microbiology* HIV Long Terminal Repeat / genetics* HIV-1 / genetics* Humans Leukocytes, Mononuclear / microbiology Lymph Nodes / microbiology Molecular Sequence Data Phylogeny* Polymerase Chain Reaction Proviruses / genetics Sequence Alignment Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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