| Phylogenetic conservation of the cell-type-specific Lan3-2 glycoepitope in Caenorhabditis elegans. | |
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MedLine Citation:
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PMID: 20563596 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The biological function of a cell-type-specific glycosylation of an adhesion molecule belonging to the L1CAM immunoglobulin superfamily was previously determined in the nervous system of the embryonic leech, Hirudo medicinalis. The Lan3-2 glycoepitope is a surface marker of sensory afferent neurons and is required for their appropriate developmental collateral branching and synaptogenesis in the CNS. The chemical structure of the Lan3-2 glycoepitope consists of beta-(1,4)-linked mannopyranose. Here, we show the conservation of the cell-type-specific expression of this mannose polymer in Caenorhabditis elegans. The Lan3-2 glycoepitope is expressed on the cell surface of a subset of dissociated embryonic neurons and, in the adult worm, by the pharyngeal motor neuron, M5, and the chemosensory afferents, the amphids. Additionally, the vulval epithelium expresses the Lan3-2 glycoepitope in late L4 larvae and in adult hermaphrodites. To investigate proteins carrying this restrictively expressed glycoepitope, worm extract was immunoaffinity purified with Lan3-2 monoclonal antibody and Western blotted. A polyclonal antibody reactive with the cytoplasmic tail of LAD-1/SAX-7, a C. elegans member of the L1CAM family, recognizes a 270 kDa protein band while Lan3-2 antibody also recognizes a 190 kDa glycoform, its putative Lan3-2 ectodomain. Thus, in C. elegans, as in leech, the Lan3-2 epitope is located on a L1CAM homologue. The cell-type-specific expression of the Lan3-2 glycoepitope shared by leech and C. elegans will be useful for understanding how cell-type-specific glycoepitopes mediate cell-cell interactions during development. |
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Authors:
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Harper C Vansteenhouse; Zachary A Horton; Robert O'Hagan; Mei-Hui Tai; Birgit Zipser |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-06-19 |
Journal Detail:
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Title: Development genes and evolution Volume: 220 ISSN: 1432-041X ISO Abbreviation: Dev. Genes Evol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9613264 Medline TA: Dev Genes Evol Country: Germany |
Other Details:
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Languages: eng Pagination: 77-87 Citation Subset: IM |
Affiliation:
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Department of Physiology, Michigan State University, East Lansing, MI 48824, USA. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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NS5117/NS/NINDS NIH HHS; //Howard Hughes Medical Institute |
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