Document Detail


Phylogenetic conservation of the cell-type-specific Lan3-2 glycoepitope in Caenorhabditis elegans.
MedLine Citation:
PMID:  20563596     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The biological function of a cell-type-specific glycosylation of an adhesion molecule belonging to the L1CAM immunoglobulin superfamily was previously determined in the nervous system of the embryonic leech, Hirudo medicinalis. The Lan3-2 glycoepitope is a surface marker of sensory afferent neurons and is required for their appropriate developmental collateral branching and synaptogenesis in the CNS. The chemical structure of the Lan3-2 glycoepitope consists of beta-(1,4)-linked mannopyranose. Here, we show the conservation of the cell-type-specific expression of this mannose polymer in Caenorhabditis elegans. The Lan3-2 glycoepitope is expressed on the cell surface of a subset of dissociated embryonic neurons and, in the adult worm, by the pharyngeal motor neuron, M5, and the chemosensory afferents, the amphids. Additionally, the vulval epithelium expresses the Lan3-2 glycoepitope in late L4 larvae and in adult hermaphrodites. To investigate proteins carrying this restrictively expressed glycoepitope, worm extract was immunoaffinity purified with Lan3-2 monoclonal antibody and Western blotted. A polyclonal antibody reactive with the cytoplasmic tail of LAD-1/SAX-7, a C. elegans member of the L1CAM family, recognizes a 270 kDa protein band while Lan3-2 antibody also recognizes a 190 kDa glycoform, its putative Lan3-2 ectodomain. Thus, in C. elegans, as in leech, the Lan3-2 epitope is located on a L1CAM homologue. The cell-type-specific expression of the Lan3-2 glycoepitope shared by leech and C. elegans will be useful for understanding how cell-type-specific glycoepitopes mediate cell-cell interactions during development.
Authors:
Harper C Vansteenhouse; Zachary A Horton; Robert O'Hagan; Mei-Hui Tai; Birgit Zipser
Related Documents :
11078926 - In vivo regulation of precursor cells in the subventricular zone of adult rat brain by ...
18088956 - A single cell analysis of engrailed expression in the early embryonic brain of the gras...
3446206 - Transplantation of dentate granular cells after lesion of the dentate area by colchicine.
19664656 - Enrichment of rat oligodendrocyte progenitor cells by magnetic cell sorting.
9856766 - Human corneal epithelial cell functional responses to inflammatory agents and their ant...
6209936 - The induction of enhanced glucosamine incorporation into the cell-envelope of escherich...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-06-19
Journal Detail:
Title:  Development genes and evolution     Volume:  220     ISSN:  1432-041X     ISO Abbreviation:  Dev. Genes Evol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9613264     Medline TA:  Dev Genes Evol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  77-87     Citation Subset:  IM    
Affiliation:
Department of Physiology, Michigan State University, East Lansing, MI 48824, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
NS5117/NS/NINDS NIH HHS; //Howard Hughes Medical Institute

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Increased P2X7R expression in atrial cardiomyocytes of caveolin-1 deficient mice.
Next Document:  Triple expression of B7-1, B7-2 and 4-1BBL enhanced antitumor immune response against mouse H22 hepa...