Document Detail


Phylogenetic analysis of near full-length HIV type 1 genomic sequences from 21 Korean individuals.
MedLine Citation:
PMID:  23199052     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Korean subclade of subtype B (KSB) is the most prevalent HIV-1 strain found in Korea. To date, only two near full-length HIV-1 sequences from Korean patients have been reported. Here, we analyzed a total of 24 near full-length genomes of HIV-1 strains that were isolated from 17 antiretroviral therapy (ART)-naive patients and four ART-exposed patients. Proviral DNA from peripheral blood mononuclear cells was PCR amplified and directly sequenced. Phylogenetic analyses were used to classify viruses from 19 patients as KSB, from one patient as subtype B, from one patient as subtype D, and three viruses from one patient as CRF02_AG. All KSB viruses demonstrated TAAAA instead of TATAA at the TATA box in the LTR. Of the 19 KSB patients, their sequence identities at the nucleotide level ranged from 89.8% to 97.1% from the lowest env gene to the highest pol gene. Other than the CRF02_AG viruses, no recombination events were noted in any of the 19 KSB patients, which is consistent with our previous studies on the pol, vif, and nef genes. Except for one strain, all of the strains were classified as non-syncytium-inducing strains. This is the first report to describe near full-length KSB.
Authors:
Young-Keol Cho; Jung-Eun Kim; Brian T Foley
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-11
Journal Detail:
Title:  AIDS research and human retroviruses     Volume:  29     ISSN:  1931-8405     ISO Abbreviation:  AIDS Res. Hum. Retroviruses     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-08     Completed Date:  2013-09-11     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  8709376     Medline TA:  AIDS Res Hum Retroviruses     Country:  United States    
Other Details:
Languages:  eng     Pagination:  738-43     Citation Subset:  IM; X    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF224507;  AY839827;  DQ054367;  DQ295192;  DQ295193;  DQ295194;  DQ295195;  DQ295196;  DQ837381;  JQ316126;  JQ316127;  JQ316128;  JQ316129;  JQ316130;  JQ316131;  JQ316132;  JQ316133;  JQ316134;  JQ316135;  JQ316136;  JQ316137;  JQ316138;  JQ341411;  JQ429433
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MeSH Terms
Descriptor/Qualifier:
Antiretroviral Therapy, Highly Active
Base Sequence
DNA, Viral / genetics
Drug Resistance, Viral / genetics
Female
Genes, env
Genes, pol
HIV Infections / drug therapy,  epidemiology,  virology*
HIV-1 / classification*,  genetics*,  isolation & purification
Humans
Male
Molecular Epidemiology
Molecular Sequence Data
Phylogeny
Recombination, Genetic
Republic of Korea / epidemiology
Sequence Homology, Nucleic Acid
TATA Box
Chemical
Reg. No./Substance:
0/DNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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