| Phyllanthus urinaria increases apoptosis and reduces telomerase activity in human nasopharyngeal carcinoma cells. | |
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MedLine Citation:
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PMID: 19295228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: This study was designed to obtain the chemical fingerprint and to investigate the effect of Phyllanthus urinaria on telomerase activity and apoptotic pathways in the human nasopharyngeal carcinoma cell line (NPC-BM1). MATERIALS AND METHODS: The polyphenol compounds in P. urinaria were investigated by HPLC/MS. Cell viability with the treatment of P. urinaria, gallic acid, ellagic acid, quercetin and cisplatin was detected by MTT assay. TUNEL assay, DNA fragmentation analysis and caspase3 activity were used to confirm apoptotic changes. Telomerase activity was determined using the TRAP assay. RNA isolation and RT-PCR were used to analyze the related genes expression. All experiments on treatments with P. urinaria from 0-3 mg/ml were carried out for 24 h. RESULTS: 5 major compounds including gallic acid, brevifolin carboxylic acid, corilagin, phyllanthusiin C and ellagic acid were identified as a plant fingerprint by HPLC/MS. With the MTT assay, we demonstrated that P. urinaria, gallic acid and ellagic acid reduce cell viability. The apoptosis features showed DNA fragmentation and increased caspase-3 activity associated with the down-regulation of Bcl-2, but not of Bax, p53, and PCNA (proliferating cell nuclear antigen) in P. urinaria-treated NPC-BM1 cells. Furthermore, treatment of NPC-BM1 cells led to an inhibition of hTERT (human telomerase reverse transcriptase), hTP1 (human telomerase-associated protein 1) and c-myc mRNA expression and to decreased telomerase activity. CONCLUSION: This study suggests that P. urinaria induces the death of NPC-BM1 cells in vitro through the induction of apoptosis and inhibited telomerase activity. |
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Authors:
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Sheng-Teng Huang; Chen-Yu Wang; Rong-Chi Yang; Chih-Ju Chu; Hsiao-Ting Wu; Jong-Hwei S Pang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-06 |
Journal Detail:
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Title: Forschende Komplementärmedizin (2006) Volume: 16 ISSN: 1661-4127 ISO Abbreviation: - Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-03-19 Completed Date: 2009-09-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101269884 Medline TA: Forsch Komplementmed Country: Switzerland |
Other Details:
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Languages: eng Pagination: 34-40 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 S. Karger AG, Basel. |
Affiliation:
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Department of Chinese Medicine, Chang Gung Memorial Hospital-Kaohsiung Medical Center, Chang Gung Institute of Technology, Taiwan, Republic of China. sheng.teng@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents
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pharmacology Antioxidants / pharmacology Apoptosis / drug effects* Cell Line, Tumor Cell Survival / drug effects Chromatography, High Pressure Liquid Cisplatin / pharmacology Drugs, Chinese Herbal / chemistry, pharmacology* Ellagic Acid / pharmacology Gallic Acid / pharmacology Gene Expression Regulation / drug effects* Genes, bcl-2 / genetics Genes, myc / genetics Humans Mass Spectrometry Nasopharyngeal Neoplasms Phyllanthus / chemistry* Plant Extracts / chemistry, pharmacology* Quercetin / pharmacology Reverse Transcriptase Polymerase Chain Reaction Telomerase |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Antioxidants; 0/Drugs, Chinese Herbal; 0/Plant Extracts; 117-39-5/Quercetin; 149-91-7/Gallic Acid; 15663-27-1/Cisplatin; 476-66-4/Ellagic Acid; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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