Document Detail


Phyllanthus amarus inhibits cell growth and induces apoptosis in Dalton's lymphoma ascites cells through activation of caspase-3 and downregulation of Bcl-2.
MedLine Citation:
PMID:  19223368     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The authors found in an earlier study that Phyllanthus amarus extract could significantly inhibit the solid and ascites tumor development in mice induced by Dalton's lymphoma ascites (DLA) cells. In the present study, the apoptotic effects of P. amarus against DLA cells in culture was evaluated. P. amarus produced significant reduction in cell viability as determined by the MTT assay. It also induces the formation of apoptotic bodies with characteristic features like plasma membrane invagination, elongation, fragmentation, and chromatin condensation. P. amarus at concentrations of 100 and 200 microg/mL is shown to induce DNA fragmentation. Gene expression analysis reveals that P. amarus induces the expression of caspase-3 and inhibits the expression of Bcl-2, which is an antiapoptotic protein. So the present study provides some insights into the possible mechanism by which P. amarus brings about apoptosis and growth inhibition in DLA cells.
Authors:
Kuzhuvelil B Harikumar; Girija Kuttan; Ramadasan Kuttan
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Publication Detail:
Type:  Journal Article     Date:  2009-02-17
Journal Detail:
Title:  Integrative cancer therapies     Volume:  8     ISSN:  1534-7354     ISO Abbreviation:  Integr Cancer Ther     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-08-14     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128834     Medline TA:  Integr Cancer Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  190-4     Citation Subset:  IM    
Affiliation:
Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India. kbharikumar@gmail .com
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Ascites / pathology
Caspase 3 / genetics*
Cell Line, Tumor
Cell Proliferation / drug effects
DNA Fragmentation / drug effects
Down-Regulation / drug effects*,  genetics
Enzyme Induction / drug effects
Humans
Lymphoma / metabolism,  pathology*
Phyllanthus / chemistry*
Plant Extracts / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / genetics*
Chemical
Reg. No./Substance:
0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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