| Phyllanthus amarus inhibits cell growth and induces apoptosis in Dalton's lymphoma ascites cells through activation of caspase-3 and downregulation of Bcl-2. | |
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MedLine Citation:
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PMID: 19223368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The authors found in an earlier study that Phyllanthus amarus extract could significantly inhibit the solid and ascites tumor development in mice induced by Dalton's lymphoma ascites (DLA) cells. In the present study, the apoptotic effects of P. amarus against DLA cells in culture was evaluated. P. amarus produced significant reduction in cell viability as determined by the MTT assay. It also induces the formation of apoptotic bodies with characteristic features like plasma membrane invagination, elongation, fragmentation, and chromatin condensation. P. amarus at concentrations of 100 and 200 microg/mL is shown to induce DNA fragmentation. Gene expression analysis reveals that P. amarus induces the expression of caspase-3 and inhibits the expression of Bcl-2, which is an antiapoptotic protein. So the present study provides some insights into the possible mechanism by which P. amarus brings about apoptosis and growth inhibition in DLA cells. |
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Authors:
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Kuzhuvelil B Harikumar; Girija Kuttan; Ramadasan Kuttan |
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Publication Detail:
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Type: Journal Article Date: 2009-02-17 |
Journal Detail:
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Title: Integrative cancer therapies Volume: 8 ISSN: 1534-7354 ISO Abbreviation: Integr Cancer Ther Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-08-14 Completed Date: 2009-09-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128834 Medline TA: Integr Cancer Ther Country: United States |
Other Details:
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Languages: eng Pagination: 190-4 Citation Subset: IM |
Affiliation:
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Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India. kbharikumar@gmail .com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Ascites / pathology Caspase 3 / genetics* Cell Line, Tumor Cell Proliferation / drug effects DNA Fragmentation / drug effects Down-Regulation / drug effects*, genetics Enzyme Induction / drug effects Humans Lymphoma / metabolism, pathology* Phyllanthus / chemistry* Plant Extracts / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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