Document Detail


Phthalic acid mimics 17beta-estradiol actions in WISH cells.
MedLine Citation:
PMID:  11137322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The object of this study was to evaluate whether phthalic acid, which is one of the metabolites of phthalic acid esters, exerts estrogenic actions in WISH cells, an immortalized cell line derived from human amniotic tissue. Our data demonstrate that phthalic acid (i) displaces [3H]estradiol from its binding sites, (ii) enhances the intracellular cyclic AMP concentration, without influencing adenylyl cyclase activity, (iii) stimulates or inhibits prostaglandin output, probably depending on the intracellular nucleotide level. The effects on prostanoid release are counteracted by addition of the protein-synthesis inhibitor cycloheximide, or when the diffusion of phthalic acid through the cell membrane is prevented. On the basis of our previous demonstration, that 17beta-estradiol exerts similar effects in WISH cells, we suggest that the molecular mechanisms underlying phthalic acid and steroid-hormone responses in this cell line are the same. This is the first demonstration that phthalic acid binds to the estrogen receptor with high affinity and mimics the hormone physiological actions.
Authors:
B Pavan; C Biondi; M E Ferretti; L Lunghi; G Paganetto
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology letters     Volume:  118     ISSN:  0378-4274     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-09     Completed Date:  2001-01-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Affiliation:
Section of General Physiology, Department of Biology, University of Ferrara, via Luigi Borsari 46, 44100-I, Ferrara, Italy.
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MeSH Terms
Descriptor/Qualifier:
3',5'-Cyclic-AMP Phosphodiesterases / antagonists & inhibitors
4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / pharmacology
Amnion / drug effects*,  metabolism,  secretion
Binding Sites
Binding, Competitive
Cell Line
Cyclic AMP / metabolism
Dinoprostone / secretion
Estradiol / metabolism,  pharmacology*,  physiology
Humans
Kinetics
Molecular Mimicry
Phosphodiesterase Inhibitors / pharmacology
Phthalic Acids / metabolism,  toxicity*
Receptors, Estradiol / metabolism
Chemical
Reg. No./Substance:
0/Phosphodiesterase Inhibitors; 0/Phthalic Acids; 0/Receptors, Estradiol; 29925-17-5/4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; 363-24-6/Dinoprostone; 50-28-2/Estradiol; 60-92-4/Cyclic AMP; 88-99-3/phthalic acid; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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