Document Detail


Phototropin involvement in the expression of genes encoding chlorophyll and carotenoid biosynthesis enzymes and LHC apoproteins in Chlamydomonas reinhardtii.
MedLine Citation:
PMID:  16972865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phototropin (PHOT) is a photoreceptor involved in a variety of blue-light-elicited physiological processes including phototropism, chloroplast movement and stomatal opening in plants. The work presented here tests whether PHOT is involved in expression of light-regulated genes in Chlamydomonas reinhardtii. When C. reinhardtii was transferred from the dark to very low-fluence rate white light, there was a substantial increase in the level of transcripts encoding glutamate-1-semialdehyde aminotransferase (GSAT), phytoene desaturase (PDS) and light-harvesting polypeptides (e.g. LHCBM6). Increased levels of these transcripts were also elicited by low-intensity blue light, and this blue-light stimulation was suppressed in three different RNAi strains that synthesize low levels of PHOT. The levels of GSAT and LHCBM6 transcripts also increased following exposure of algal cells to low-intensity red light (RL). The red-light-dependent increase in transcript abundance was not affected by the electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, implying that the influence of RL on transcript accumulation was not controlled by cytoplasmic redox conditions, and that a red-light photoreceptor(s) may be involved in regulating the levels of transcripts from specific photosynthesis-related genes in C. reinhardtii. Interestingly, elevated GSAT and LHCBM6 transcript levels in RL were significantly reduced in the PHOT RNAi strains, which raises the possibility of co-action between blue and RL signaling pathways. Microarray experiments indicated that the levels of several transcripts for photosystem (PS) I and II polypeptides were also modulated by PHOT. These data suggest that, in C. reinhardtii, (i) PHOT is involved in blue-light-mediated changes in transcript accumulation, (ii) synchronization of the synthesis of chlorophylls (Chl), carotenoids, Chl-binding proteins and other components of the photosynthetic apparatus is achieved, at least in part, through PHOT-mediated signaling, and (iii) a red-light photoreceptor can also influence levels of certain transcripts associated with photosynthetic function, although its action requires normal levels of PHOT.
Authors:
Chung-Soon Im; Stephan Eberhard; Kaiyao Huang; Christoph F Beck; Arthur R Grossman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  48     ISSN:  0960-7412     ISO Abbreviation:  Plant J.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-15     Completed Date:  2006-12-18     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Affiliation:
Department of Plant Biology, Carnegie Institution, Stanford, CA 94306, USA. csim@stanford.edu
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MeSH Terms
Descriptor/Qualifier:
Algal Proteins / genetics,  metabolism*
Animals
Apoproteins / genetics,  metabolism
Carotenoids / biosynthesis*
Chlamydomonas reinhardtii / enzymology,  genetics,  metabolism*
Chlorophyll / biosynthesis*
Cryptochromes
Diuron / pharmacology
Electron Transport / drug effects
Flavoproteins / physiology*
Gene Expression Regulation
Intramolecular Transferases / genetics,  metabolism
Light
Light-Harvesting Protein Complexes / genetics,  metabolism
Oligonucleotide Array Sequence Analysis
Oxidoreductases / genetics,  metabolism
Photosynthesis
Phototropism
RNA Interference
RNA, Messenger / metabolism
Signal Transduction / physiology
Chemical
Reg. No./Substance:
0/Algal Proteins; 0/Apoproteins; 0/Cryptochromes; 0/Flavoproteins; 0/Light-Harvesting Protein Complexes; 0/RNA, Messenger; 1406-65-1/Chlorophyll; 330-54-1/Diuron; 36-88-4/Carotenoids; EC 1.-/Oxidoreductases; EC 1.14.99.-/phytoene dehydrogenase; EC 5.4.-/Intramolecular Transferases; EC 5.4.3.8/glutamate-1-semialdehyde 2,1-aminomutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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