Document Detail


Phototransformations of advanced glycation end products in the human eye lens due to ultraviolet A light irradiation.
MedLine Citation:
PMID:  16037236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies from this laboratory have shown that ultraviolet A (UVA) light can bleach the yellow advanced glycation end products (AGEs) of aged and cataractous human lenses. The AGEs OP-lysine and argpyrimidine are two UVA-absorbing posttranslational modifications that are abundant in the eye lens. The purpose of this study was to outline the changes in these two AGEs due to UVA irradiation. The changes of OP-lysine, OP-phenethylamine (a phenethylamine analogue of OP-lysine), and argpyrimidine due to irradiation with UVA light in the presence or absence of air and ascorbic acid were followed by different spectral methods. Aged human lenses were similarly irradiated in artificial aqueous humor. The amounts of OP-lysine in the irradiated lenses and in the corresponding dark controls were determined by HPLC. Both OP-lysine and argpyrimidine decreased 20% when irradiated with UVA light in the absence of ascorbic acid. Under the same conditions, OP-lysine was bleached 80% in the presence of ascorbic acid during irradiation experiments. In contrast, argpyrimidine UVA light bleaching was not affected by the presence of ascorbic acid. Interestingly the major product of OP-phenethylamine after UVA irradiation in the presence of ascorbic acid was phenethylamine, which indicates that the entire heterocycle of this AGE was cleaved and the initial amino group was restored. Some AGEs in the human eye lens can be transformed by UVA light.
Authors:
O K Argirov; B Lin; B J Ortwerth
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1043     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-22     Completed Date:  2005-10-27     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  166-73     Citation Subset:  IM    
Affiliation:
Mason Eye Institute-East, University of Missouri at Columbia, 404 Portland Street, Columbia, MO 65201, USA. argirovo@health.missouri.edu
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MeSH Terms
Descriptor/Qualifier:
Glycosylation End Products, Advanced / radiation effects*
Humans
Lens, Crystalline / physiology*,  radiation effects
Lysine / analogs & derivatives,  radiation effects
Protein Processing, Post-Translational
Pyridinium Compounds / radiation effects
Ultraviolet Rays*
Grant Support
ID/Acronym/Agency:
EY 07070/EY/NEI NIH HHS; EY02035/EY/NEI NIH HHS; R01 EY002035-28/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/2-amino-6-(3-oxidopyridinium-1-yl)hexanoic acid; 0/Glycosylation End Products, Advanced; 0/Pyridinium Compounds; 56-87-1/Lysine
Comments/Corrections

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