| Phototoxicity of skin microorganisms tested with a new model. | |
| | |
MedLine Citation:
|
PMID: 3288127 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A new standardized method for testing phototoxicity of chemicals against microorganisms is described. The inoculum size of the microorganism, application of test chemicals, prediffusion time, incubation time and incubation period are defined. Staphylococcus aureus, S. epidermidis, Candida albicans, and Pityrosporum orbiculare were studied. Both 8-methoxypsoralen and trimethylpsoralen were phototoxic against all microorganisms tested, while tetracycline and doxycycline were not phototoxic. C. albicans may be chosen for phototoxicity testing because it has been used earlier, it is easy to maintain in culture, it grows easily when tested, and its pathogenicity is low. The phototoxicity of S. aureus, S. epidermidis, Pseudomonas aeruginosa, Propionibacterium acnes, C. albicans, and P. orbiculare against each other were also investigated. Only P. orbiculare was inhibitory. It inhibited the growth of S. aureus, S. epidermidis, and Ps. aeruginosa - both in the dark and after irradiation. The growth inhibition was markedly enhanced after UVA irradiation, indicating phototoxicity. The phototoxic effect of P. orbiculare may play a role in the ecology of the human skin flora. |
| | |
Authors:
|
J Faergemann; O Larkö |
Related Documents
:
|
1774297 - Reproducibility of tolerance tests that are useful in the identification of campylobact... 1084727 - Characterization of an ampicillin-resistant haemophilus influenzae type b. 20819577 - Comparison of a glucose consumption based method with the clsi m38-a method for testing... 17728477 - Comparison between disk diffusion and microdilution methods for determining susceptibil... 22544187 - Evaluation of in-house genotyping assay performance using dried blood spot specimens in... 19208887 - Comparative evaluation of three commercial toxoplasma-specific igg antibody avidity tes... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Archives of dermatological research Volume: 280 ISSN: 0340-3696 ISO Abbreviation: Arch. Dermatol. Res. Publication Date: 1988 |
Date Detail:
|
Created Date: 1988-07-11 Completed Date: 1988-07-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8000462 Medline TA: Arch Dermatol Res Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 168-70 Citation Subset: IM |
Affiliation:
|
Department of Dermatology, University of Gothenburg, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacteria
/
drug effects*,
radiation effects Candida / drug effects*, radiation effects Malassezia / drug effects*, radiation effects Methoxsalen / pharmacology* Microbial Sensitivity Tests Microbiological Techniques* Photochemistry Psoralens / pharmacology* Skin / microbiology* Tetracycline / pharmacology* Trioxsalen / pharmacology* Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
|
0/Psoralens; 298-81-7/Methoxsalen; 3902-71-4/Trioxsalen; 60-54-8/Tetracycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunohistochemical localization of type V collagen in normal human skin.
Next Document: Exposure assessment: input into risk assessment.