Document Detail

Phototoxic effects of 635-nm light on canine transitional cell carcinoma cells incubated with 5-aminolevulinic acid.
MedLine Citation:
PMID:  12602579     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To determine whether transitional cell carcinoma (TCC) cells incubated in media containing 5-aminolevulinic acid (ALA) would produce sufficient protoporphyrin IX (PpIX) to cause lethal phototoxic effects when exposed to 635-nm light. SAMPLE POPULATION: Canine TCC cells (K9TCC). PROCEDURE: Cultured K9TCC cells were exposed to graded doses of ALA, and PpIX concentrations were determined. Cells then were exposed to various doses of 635-nm light from a diode laser, and cell viability was assayed. RESULTS: Production of PpIX was dependent on time and dose of ALA. The K9TCC cells incubated with ALA produced sufficient PpIX to cause lethal phototoxic effects when exposed to 635-nm light. Phototoxic effects were dependent on time and dose of ALA. Increasing laser power density and energy density decreased cell survival. CONCLUSIONS AND CLINICAL RELEVANCE: ALA is an effective photosensitizer for in vitro photodynamic treatment of K9TCC cells. Further studies are warranted to assess the safety and efficacy of ALA as a photosensitizer for use in treating dogs with TCC. Impact for Human Medicine-On the basis of this study, dogs with TCC may be useful in the development of protocols for ALA-based photodynamic therapy of humans affected with muscle-invasive bladder cancer.
Tisha D Ridgway; Michael D Lucroy
Related Documents :
18855599 - Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (asgpr) expressi...
17518439 - Porphyrin-retinamides: synthesis and cellular studies.
20732049 - The effect of desferrioxamine on cell proliferation in human tumour cell lines.
750549 - Hepatic sinusoidal cells in iron overload. ultrastructural observations.
15629649 - Kinetics of mtt-formazan exocytosis in phagocytic and non-phagocytic cells.
9253029 - Direct evidence that iron deprivation induces apoptosis in murine lymphoma 38c13.
9672699 - The characterization of cell death induced by 1-(3-c-ethynyl-beta-d-ribo-pentofuranosyl...
19207549 - Influence of aspirin and cigarette smoke extract on the expression of cyclin d1 and eff...
16600019 - Effects of quercetin on insulin-like growth factors (igfs) and their binding protein-3 ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of veterinary research     Volume:  64     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-26     Completed Date:  2003-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-6     Citation Subset:  IM    
Comparative Biophotonics Laboratory, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Aminolevulinic Acid / pharmacology*
Carcinoma, Transitional Cell / drug therapy*,  metabolism,  pathology,  veterinary*
Cell Survival / drug effects,  radiation effects
Dog Diseases / drug therapy*,  metabolism,  pathology*
Dose-Response Relationship, Drug
Protoporphyrins / metabolism
Time Factors
Tumor Cells, Cultured
Reg. No./Substance:
0/Protoporphyrins; 106-60-5/Aminolevulinic Acid; 553-12-8/protoporphyrin IX

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Low biochemical nutritional parameters in acutely ill hospitalized elderly patients with and without...
Next Document:  Comparison of electroacupuncture and butorphanol on respiratory and cardiovascular effects and recta...