| Phototoxic effects of 635-nm light on canine transitional cell carcinoma cells incubated with 5-aminolevulinic acid. | |
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MedLine Citation:
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PMID: 12602579 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To determine whether transitional cell carcinoma (TCC) cells incubated in media containing 5-aminolevulinic acid (ALA) would produce sufficient protoporphyrin IX (PpIX) to cause lethal phototoxic effects when exposed to 635-nm light. SAMPLE POPULATION: Canine TCC cells (K9TCC). PROCEDURE: Cultured K9TCC cells were exposed to graded doses of ALA, and PpIX concentrations were determined. Cells then were exposed to various doses of 635-nm light from a diode laser, and cell viability was assayed. RESULTS: Production of PpIX was dependent on time and dose of ALA. The K9TCC cells incubated with ALA produced sufficient PpIX to cause lethal phototoxic effects when exposed to 635-nm light. Phototoxic effects were dependent on time and dose of ALA. Increasing laser power density and energy density decreased cell survival. CONCLUSIONS AND CLINICAL RELEVANCE: ALA is an effective photosensitizer for in vitro photodynamic treatment of K9TCC cells. Further studies are warranted to assess the safety and efficacy of ALA as a photosensitizer for use in treating dogs with TCC. Impact for Human Medicine-On the basis of this study, dogs with TCC may be useful in the development of protocols for ALA-based photodynamic therapy of humans affected with muscle-invasive bladder cancer. |
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Authors:
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Tisha D Ridgway; Michael D Lucroy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: American journal of veterinary research Volume: 64 ISSN: 0002-9645 ISO Abbreviation: Am. J. Vet. Res. Publication Date: 2003 Feb |
Date Detail:
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Created Date: 2003-02-26 Completed Date: 2003-06-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375011 Medline TA: Am J Vet Res Country: United States |
Other Details:
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Languages: eng Pagination: 131-6 Citation Subset: IM |
Affiliation:
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Comparative Biophotonics Laboratory, Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aminolevulinic Acid
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pharmacology* Animals Carcinoma, Transitional Cell / drug therapy*, metabolism, pathology, veterinary* Cell Survival / drug effects, radiation effects Dog Diseases / drug therapy*, metabolism, pathology* Dogs Dose-Response Relationship, Drug Lasers Light* Photochemotherapy Protoporphyrins / metabolism Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Protoporphyrins; 106-60-5/Aminolevulinic Acid; 553-12-8/protoporphyrin IX |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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