| Photothermal detection of nicotine-induced apoptotic effects in pancreatic cancer cells. | |
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MedLine Citation:
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PMID: 15369703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this study was to evaluate the capability of a photothermal (PT) assay in determining the effects of graded doses of nicotine in a pancreatic acinar cell line (AR42J). The cellular response to nicotine was detected through the monitoring of PT signals from light-absorbing endogenous cellular structures that have been used as natural indicators for nicotine's action. It was demonstrated that introducing nicotine to cultured acinar cells in vitro leads to changes in cellular absorbing structures, thereby altering the microstructure of PT cell images and the temporal shape of PT signals. The results showed that the dependence of specific PT parameters was almost proportional to nicotine concentrations ranging from 1 nM to 100 microM, with the saturation maximum at and around 100 microM - 1 mM; thereafter, PT signals decreased rapidly to control levels and even lower, in the range of 1 - 50 mM. Conventional fluorescent tests (Annexin V--Propidium Iodide) performed in parallel showed no effect with nicotine at a concentration <1 microM (three orders of magnitude greater than the sensitivity threshold of the PT assay). With an increase in nicotine concentration from 1 mM to 50 mM, rapidly growing apoptotic and necrotic cells were detected. Thus, the PT assay demonstrated the capability for high-sensitivity detection of nicotine's impact, which may be related to a change in cell metabolism, apoptosis, or necrosis, depending on nicotine concentration. |
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Authors:
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Valentin Galitovsky; Parimal Chowdhury; Vladimir P Zharov |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Life sciences Volume: 75 ISSN: 0024-3205 ISO Abbreviation: Life Sci. Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-09-16 Completed Date: 2004-10-21 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: England |
Other Details:
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Languages: eng Pagination: 2677-87 Citation Subset: IM |
Affiliation:
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Philips Classic Laser Biomedical Laboratory, University of Arkansas for Medical Sciences (UAMS), 4301 West Markham St., Slot #543, Little Rock, AR 72205, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Cell Line, Tumor Dose-Response Relationship, Drug Hot Temperature Humans Microscopy Necrosis Nicotine / pharmacology* Pancreatic Neoplasms / pathology* |
| Grant Support | |
ID/Acronym/Agency:
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R21-CA097422/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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54-11-5/Nicotine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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