| Phototaxis of Haloarcula marismortui revealed through a novel microbial motion analysis algorithm. | |
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MedLine Citation:
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PMID: 20553410 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Haloarcula marismortui has been described to be nonmotile prior to the recent identification of flagellar filaments, suggesting the motile nature of H. marismortui. Here we observed the locomotion of freshly cultured H. marismortui cells and tracked the swimming trajectories via ImageJ. Trajectories of H. marismortui are intrinsically noisy, posing difficulties in motion analysis with previously established algorithms. By introducing the concept of "window vector," a Microsoft Excel-VBA-implemented microbial motion analysis algorithm reported here was able to (1) discriminate nonswimming objects from swimming cells without empirical customization by applying a power-law relationship and (2) reduce the noise caused by Brownian motion, thus enhancing the accuracy of swim reversal identification. Based on this motion analysis algorithm, two recently identified sensory rhodopsins, HmSRI and HmSRII, were shown to mediate photoattractant and photorepellent responses, respectively, revealing the phototactic activity of H. marismortui, the only archaeon showing such phenomenon other than Halobacterium salinarum. |
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Authors:
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Yu-Cheng Lin; Hsu-Yuan Fu; Chii-Shen Yang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 86 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-11-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 1084-90 Citation Subset: IM |
Copyright Information:
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© 2010 U.S. Government. Journal Compilation. The American Society of Photobiology. |
Affiliation:
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Institute of Microbiology and Biochemistry, National Taiwan University, Taipei, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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