Document Detail


Photosystem II Photosynthetic Unit Sizes from Fluorescence Induction in Leaves : CORRELATION TO PHOTOSYNTHETIC CAPACITY.
MedLine Citation:
PMID:  16661716     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
The use of fluorescence induction measurements in leaves infiltrated with 3-(3,4-dichlorophenyl)-1,1-dimethylurea has been evaluated as a routine method for estimation of the concentration of the reaction centers of photosystem II relative to total chlorophyll in a wide variety of plant species. The procedure is based on a simple theory that takes into account the attenuation of light in passing through the leaf and the linear dependence of the fluorescence induction time from different parts of the leaf on the inverse of the local light intensity. A formula to calculate the reaction center concentration of photosystem II was obtained. The effect of the light attenuation is accounted for by a correction factor which could become practically insignificant by an optimal choice of the excitation and emission wavelengths and the geometry of the photodetector with respect to the sample. Estimation of quantum yields for primary photochemistry and influence of light scattering were considered. The results demonstrate the effect of the above factors under various circumstances and are in agreement, to a first approximation, with the theory.THE UTILITY OF THE METHOD IS DEMONSTRATED BY A DETAILED STUDY OF FOUR DESERT PLANT SPECIES: estimation of reaction center concentrations of both photosystem I (by estimation of P700) and photosystem II (by the fluorescence induction method) were made and were compared to the rates of CO(2) fixation. There was a good quantitative correlation between the photosynthetic rates and the concentration of photosystem II reaction centers (expressed as per chlorophyll or per unit area of the leaf), but no such correlation was found with photosystem I reaction centers.The ratio of total chlorophyll per reaction centers II varied in the range of about 200 to 800 in different species, but there was no variation of this parameter in any single species.
Authors:
S Malkin; P A Armond; H A Mooney; D C Fork
Related Documents :
24253516 - Stomatal opening in isolated epidermis of commelina benghalensis l. heterophasic respon...
20655756 - The photoprotective mechanisms in secale cereale leaves under cu and high light stress ...
24414576 - Chlorophyll fluorescence and photoinhibition in a tropical rainforest understory plant.
16356906 - Physiological responses of three deciduous conifers (metasequoia glyptostroboides, taxo...
406676 - Visual sensitivity: significant within-species variations in a nonhuman primate.
10927116 - Coincidence detectors: visual processing of a pair of lines and implications for shape ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  67     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1981 Mar 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  570-9     Citation Subset:  -    
Affiliation:
Department of Plant Biology, Carnegie Institution of Washington, Stanford University, Stanford, California 94305.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenoty...
Next Document:  Photosynthetic Units of Sun and Shade Plants.