Document Detail


Photosensitizing mechanism and identification of levofloxacin photoproducts at ambient UV radiation.
MedLine Citation:
PMID:  22211524     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Levofloxacin (LVFX) is a broad spectrum third generation fluoroquinolone antibiotic, used in the treatment of severe or life-threatening bacterial infections. Photosensitizing mechanism of LVFX was investigated under the ambient environmental intensities of UV-A, UV-B and sunlight exposure. Phototoxic effects of LVFX were assessed on NIH-3T3 and HaCaT cell lines. Results identified first time three photo-products of LVFX at ambient levels of UV-R by LC-MS/MS. The generation of reactive oxygen species (ROS) was investigated photochemically as well as intracellularly in HaCaT cell line. ROS were significantly quenched by specific quenchers like DABCO, NaN(3) , D-mannitol and NAC. Photosensitized LVFX caused lipid peroxidation at different concentrations. Quenching study with SOD confirms the LVFX-induced lipid photoperoxidation. Further, photocytotoxicity of LVFX showed significant reduction in cell viability by MTT and NRU assays. LVFX caused cell arrest in G2/M phases as well as induces apoptosis through ROS-dependent pathway. In addition, photosensitized LVFX also induced upregulation of p21 and Bax/Bcl-2 genes ratio. India is a tropical country and most of the human activities such as agricultural, commercial, sports, etc. take place in bright sunlight therefore, photosensitive LVFX may lead to skin/ocular disorders and immune suppression. Information is needed regarding the phototoxicity of LVFX for human safety.
Authors:
Ashish Dwivedi; Syed F Mujtaba; Hari N Kushwaha; Daoud Ali; Neera Yadav; S K Singh; Ratan S Ray
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-29
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  -     ISSN:  1751-1097     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2012-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Photochemistry and Photobiology © 2011 American Society for Photobiology.
Affiliation:
Photobiology Division, CSIR-Indian Institute of Toxicology Research, Post Box No. 80 Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, M.G. Marg, Lucknow, 226001, India.
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