| Photosensitizing mechanism and identification of levofloxacin photoproducts at ambient UV radiation. | |
| | |
MedLine Citation:
|
PMID: 22211524 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Levofloxacin (LVFX) is a broad spectrum third generation fluoroquinolone antibiotic, used in the treatment of severe or life-threatening bacterial infections. Photosensitizing mechanism of LVFX was investigated under the ambient environmental intensities of UV-A, UV-B and sunlight exposure. Phototoxic effects of LVFX were assessed on NIH-3T3 and HaCaT cell lines. Results identified first time three photo-products of LVFX at ambient levels of UV-R by LC-MS/MS. The generation of reactive oxygen species (ROS) was investigated photochemically as well as intracellularly in HaCaT cell line. ROS were significantly quenched by specific quenchers like DABCO, NaN(3) , D-mannitol and NAC. Photosensitized LVFX caused lipid peroxidation at different concentrations. Quenching study with SOD confirms the LVFX-induced lipid photoperoxidation. Further, photocytotoxicity of LVFX showed significant reduction in cell viability by MTT and NRU assays. LVFX caused cell arrest in G2/M phases as well as induces apoptosis through ROS-dependent pathway. In addition, photosensitized LVFX also induced upregulation of p21 and Bax/Bcl-2 genes ratio. India is a tropical country and most of the human activities such as agricultural, commercial, sports, etc. take place in bright sunlight therefore, photosensitive LVFX may lead to skin/ocular disorders and immune suppression. Information is needed regarding the phototoxicity of LVFX for human safety. |
| | |
Authors:
|
Ashish Dwivedi; Syed F Mujtaba; Hari N Kushwaha; Daoud Ali; Neera Yadav; S K Singh; Ratan S Ray |
Related Documents
:
|
14600154 - Response of human pulmonary epithelial cells to lipopolysaccharide involves toll-like r... 16738534 - Reduced expression of toll-like receptor 4 contributes to impaired cytokine response of... 10623794 - Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like re... 16714144 - Elevated circulating levels of heat shock protein 70 are related to systemic inflammato... 10971784 - Infections as a major preventable cause of human cancer. 14600154 - Response of human pulmonary epithelial cells to lipopolysaccharide involves toll-like r... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-12-29 |
Journal Detail:
|
Title: Photochemistry and photobiology Volume: - ISSN: 1751-1097 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
|
Created Date: 2012-1-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Photochemistry and Photobiology © 2011 American Society for Photobiology. |
Affiliation:
|
Photobiology Division, CSIR-Indian Institute of Toxicology Research, Post Box No. 80 Pharmacokinetics and Metabolism Division, CSIR-Central Drug Research Institute, M.G. Marg, Lucknow, 226001, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Survival Characteristics and Prognostic Variables of Dogs with Preclinical Chronic Degenerative Mitr...
Next Document: Maintaining equilibrium in professional role identity: a grounded theory study of health visitors' p...