Document Detail

Photosensitizer effects on cancerous cells: a combined study using synchrotron infrared and fluorescence microscopies.
MedLine Citation:
PMID:  18342635     Owner:  NLM     Status:  MEDLINE    
Hypocrellin A (HA), a lipid-soluble peryloquinone derivative, isolated from natural fungus sacs of Hypocrella bambusae, has been reported to be a highly potential photosensitizer in photodynamic therapy (PDT). It has been studied increasingly because of its anticancer activities when irradiated with light. We have studied the interaction mechanisms of HA with HeLa cells as a function of incubation time. Fluorescence microscopy confirmed that HA localisation is limited in the cytoplasm before eventually concentrating in clusters around the nucleus. The IR spectra of HA-treated, PDT-treated and control HeLa cells were recorded at the ESRF Infrared beamline (ID21). Principal component analysis has been used to assess the IR spectral changes between the various HeLa cells spectral data sets (The Unscrambler software, CAMO). PCA revealed that there is a frequency shift of protein amide I and amide II vibrational bands, indicating changes in the protein secondary structures of the HA-treated and PDT-treated cancer cells compared to the control cells. In addition, the relative DNA intensity in HA-treated cells decreases gradually along the incubation time. The use of synchrotron infrared microscopy is shown to be of paramount importance for targeting specifically the biochemical modification induced in the cell nucleus.
Sirinart Chio-Srichan; Matthieu Réfrégiers; Frédéric Jamme; Slavka Kascakova; Valérie Rouam; Paul Dumas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-10
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1780     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  854-60     Citation Subset:  IM    
Synchrotron SOLEIL, L'Orme des Merisiers, Saint-Aubin, 91192 Gif-sur-Yvette, France.
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MeSH Terms
Cell Nucleus / drug effects,  metabolism,  radiation effects
Cytoplasm / drug effects,  metabolism,  radiation effects
Hela Cells
Infrared Rays
Intracellular Space / chemistry,  metabolism
Microscopy / methods*
Microscopy, Fluorescence / methods
Molecular Structure
Neoplasms / drug therapy,  metabolism,  pathology
Perylene / analogs & derivatives,  chemistry,  metabolism,  pharmacology
Photosensitizing Agents / chemistry,  metabolism,  pharmacology*
Principal Component Analysis
Quinones / chemistry,  metabolism,  pharmacology
Spectrophotometry, Infrared
Reg. No./Substance:
0/Photosensitizing Agents; 0/Quinones; 198-55-0/Perylene; 77029-83-5/hypocrellin A

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