| Photosensitized production of singlet oxygen: spatially-resolved optical studies in single cells. | |
| | |
MedLine Citation:
|
PMID: 19337656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Singlet molecular oxygen, O(2)(a(1)Delta(g)), can be created in photosensitized experiments with sub-cellular spatial resolution in a single cell. This cytotoxic species can subsequently be detected by its 1270 nm phosphorescence (a(1)Delta(g)--> X(3)Sigma). Cellular responses to the creation of singlet oxygen can be monitored using viability assays. Time- and spatially-resolved optical measurements of both singlet oxygen and its precursor, the excited state sensitizer, reflect the complex and dynamic morphology of the cell. These experiments help elucidate photoinduced, oxygen-dependent events that compromise cell function and ultimately lead to cell death. In this perspective, recent work on the photosensitized production and detection of singlet oxygen in single cells is summarized, highlighting the advantages and current limitations of this unique experimental approach to study an old problem. |
| | |
Authors:
|
Thomas Breitenbach; Marina K Kuimova; Peter Gbur; Sonja Hatz; Nickolass Bitsch Schack; Brian Wett Pedersen; John D C Lambert; Lars Poulsen; Peter R Ogilby |
Related Documents
:
|
18588206 - Diosgenin formation by freely suspended and entrapped plant cell cultures of dioscorea ... 10660466 - A cell viability assay based on monitoring respiration by optical oxygen sensing. 11597896 - Et-1 stimulates pulmonary arterial smooth muscle cell proliferation via induction of re... 8390246 - A new sensitive chemiluminescence probe, l-012, for measuring the production of superox... 20707646 - Differentiation of k562 cells under elf-emf applied at different time courses. 19686286 - Adenovirus vector-mediated upregulation of spermidine /spermine n1-acetyltransferase im... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-23 |
Journal Detail:
|
Title: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Volume: 8 ISSN: 1474-905X ISO Abbreviation: Photochem. Photobiol. Sci. Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-04-01 Completed Date: 2009-07-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101124451 Medline TA: Photochem Photobiol Sci Country: England |
Other Details:
|
Languages: eng Pagination: 442-52 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, University of Aarhus, DK-8000, Arhus, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Hela Cells
/
cytology*,
physiology Humans Luminescent Measurements Microscopy, Fluorescence / methods Neurons / cytology*, physiology* Perylene / analogs & derivatives, metabolism Photons Photosensitizing Agents / diagnostic use Singlet Oxygen / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Photosensitizing Agents; 17778-80-2/Singlet Oxygen; 198-55-0/Perylene; 548-04-9/hypericin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Magnifying endoscopy and chromoendoscopy of the upper gastrointestinal tract.
Next Document: Spatial and temporal dynamics of in vitro photodynamic cell killing: extracellular hydrogen peroxide...