Document Detail


Photosensitized oxidation of tryptophan: effect on liver and brain tryptophan.
MedLine Citation:
PMID:  1684996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Toxic products are formed when tryptophan is irradiated with light in the presence of photosensitizers such as riboflavin. In order to further investigate this phenomenon, solutions of tryptophan (48 mumol.mL-1) were irradiated with broad spectrum fluorescent light in the presence or absence of riboflavin (0.01 mg.mL-1). Solutions of riboflavin were similarly irradiated; control solutions were the respective solutions not exposed to light. Two-week-old suckling gerbils were then assigned to receiving 7 days of intraperitoneal injections of the light-exposed or non-light-exposed solutions. There were significant differences in the concentrations of tryptophan in serum, liver, and brain; activity of gamma-glutamyl transferase (GGT) as well as liver protein were also significantly different among the groups. Body and liver weights were also significantly different among the groups. In order to identify the photoproducts responsible for these changes, solutions of tryptophan that had been irradiated with light in the presence of riboflavin were then fractionated based on time of elution during high-pressure liquid chromatography and the fractions were then injected into the gerbils as before. GGT responses to one of the fractions was similar to that of the parent compound. Chromatographic studies indicated the presence of numerous photoadduct compounds of tryptophan and riboflavin after exposure to light. Both the presence of riboflavin and the exposure of the solutions to light alter brain concentrations of tryptophan in the developing gerbil indicating differing availability to the brain of this serotonin precursor. Clinical implications of the infusion of amino acids in the presence of photosensitizers and light must be considered.
Authors:
J Bhatia; D K Rassin; D J McAdoo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  JPEN. Journal of parenteral and enteral nutrition     Volume:  15     ISSN:  0148-6071     ISO Abbreviation:  JPEN J Parenter Enteral Nutr     Publication Date:    1991 Nov-Dec
Date Detail:
Created Date:  1992-02-20     Completed Date:  1992-02-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7804134     Medline TA:  JPEN J Parenter Enteral Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  637-41     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Texas Medical Branch, Galveston.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Weight / drug effects
Brain / drug effects,  metabolism*
Gerbillinae
Light*
Liver / anatomy & histology,  drug effects,  metabolism*
Organ Size / drug effects
Oxidation-Reduction
Photochemistry
Riboflavin / pharmacology*
Solutions
Tryptophan / chemistry,  metabolism,  pharmacology*
gamma-Glutamyltransferase / metabolism
Grant Support
ID/Acronym/Agency:
HD 22828/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Solutions; 73-22-3/Tryptophan; 83-88-5/Riboflavin; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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