Document Detail

Photorepair prevents ultraviolet-induced apoptosis in human cells expressing the marsupial photolyase gene.
MedLine Citation:
PMID:  10811124     Owner:  NLM     Status:  MEDLINE    
Photolyase absorbs blue light and employs the energy to remove UV-induced DNA damage, cyclobutane pyrimidine dimers, or pyrimidine pyrimidone (6-4) lesions. These enzymes have been found in many living organisms ranging from bacteria to aplacental mammals, but their photoreactivation effect, such as survival increase of UV-irradiated cells by light-illumination, has not been identified in placental mammals, including humans. Therefore, we introduced a photolyase gene derived from the marsupial rat kangaroo, Potorous tridactylus, into HeLa cells and established the first human cell line capable of photorepairing UV-induced pyrimidine dimers. Several clones were found to increase cell survival after UV irradiation when illuminated by fluorescent light. The induction of apoptosis by UV irradiation was investigated in these photoreactivation-proficient cells. Several typical features of the programmed cell death, such as internucleosomal DNA degradation, presence of subdiploid cells, loss of membrane integrity, and chromosomal condensation, were found to be induced by UV in the HeLa cells, but they can be reduced by photorepair. This implicates that cyclobutane pyrimidine dimers cause UV-induced apoptosis in human cells.
V Chiganças; E N Miyaji; A R Muotri; J de Fátima Jacysyn; G P Amarante-Mendes; A Yasui; C F Menck
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  60     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-06-01     Completed Date:  2000-06-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2458-63     Citation Subset:  IM    
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.
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MeSH Terms
Cell Survival / radiation effects
DNA / radiation effects
DNA Fragmentation
DNA Repair*
Deoxyribodipyrimidine Photo-Lyase / genetics*
Dose-Response Relationship, Radiation
Flow Cytometry
Hela Cells
Macropodidae / genetics*
Microscopy, Fluorescence
Pyrimidine Dimers
Time Factors
Ultraviolet Rays*
Reg. No./Substance:
0/Pyrimidine Dimers; 9007-49-2/DNA; EC Photo-Lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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