Document Detail


Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings.
MedLine Citation:
PMID:  23015764     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Photocrosslinkable, protein-engineered biomaterials combine a rapid, controllable, cytocompatible crosslinking method with a modular design strategy to create a new family of bioactive materials. These materials have a wide range of biomedical applications, including the development of bioactive implant coatings, drug delivery vehicles, and tissue engineering scaffolds. We present the successful functionalization of a bioactive elastin-like protein with photoreactive diazirine moieties. Scalable synthesis is achieved using a standard recombinant protein expression host followed by site-specific modification of lysine residues with a heterobifunctional N-hydroxysuccinimide ester-diazirine crosslinker. The resulting biomaterial is demonstrated to be processable by spin coating, drop casting, soft lithographic patterning, and mold casting to fabricate a variety of two- and three-dimensional photocrosslinked biomaterials with length scales spanning the nanometer to millimeter range. Protein thin films proved to be highly stable over a three-week period. Cell-adhesive functional domains incorporated into the engineered protein materials were shown to remain active post-photo-processing. Human adipose-derived stem cells achieved faster rates of cell adhesion and larger spread areas on thin films of the engineered protein compared to control substrates. The ease and scalability of material production, processing versatility, and modular bioactive functionality make this recombinantly engineered protein an ideal candidate for the development of novel biomaterial coatings, films, and scaffolds.
Authors:
Jordan Raphel; Andreina Parisi-Amon; Sarah Heilshorn
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Journal of materials chemistry     Volume:  22     ISSN:  0959-9428     ISO Abbreviation:  J Mater Chem     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889099     Medline TA:  J Mater Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  19429-19437     Citation Subset:  -    
Affiliation:
Department of Materials Science & Engineering, Stanford University. 476 Lomita Mall, McCullough Building 246, Stanford, CA 94035, USA.
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