| Photoprotective energy dissipation involves the reorganization of photosystem II light-harvesting complexes in the grana membranes of spinach chloroplasts. | |
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MedLine Citation:
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PMID: 21498680 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plants must regulate their use of absorbed light energy on a minute-by-minute basis to maximize the efficiency of photosynthesis and to protect photosystem II (PSII) reaction centers from photooxidative damage. The regulation of light harvesting involves the photoprotective dissipation of excess absorbed light energy in the light-harvesting antenna complexes (LHCs) as heat. Here, we report an investigation into the structural basis of light-harvesting regulation in intact spinach (Spinacia oleracea) chloroplasts using freeze-fracture electron microscopy, combined with laser confocal microscopy employing the fluorescence recovery after photobleaching technique. The results demonstrate that formation of the photoprotective state requires a structural reorganization of the photosynthetic membrane involving dissociation of LHCII from PSII and its aggregation. The structural changes are manifested by a reduced mobility of LHC antenna chlorophyll proteins. It is demonstrated that these changes occur rapidly and reversibly within 5 min of illumination and dark relaxation, are dependent on ΔpH, and are enhanced by the deepoxidation of violaxanthin to zeaxanthin. |
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Authors:
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Matthew P Johnson; Tomasz K Goral; Christopher D P Duffy; Anthony P R Brain; Conrad W Mullineaux; Alexander V Ruban |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-15 |
Journal Detail:
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Title: The Plant cell Volume: 23 ISSN: 1532-298X ISO Abbreviation: Plant Cell Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-05-27 Completed Date: 2011-09-19 Revised Date: 2012-04-02 |
Medline Journal Info:
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Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1468-79 Citation Subset: IM |
Affiliation:
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School of Biological and Chemical Sciences, Queen Mary University of London, London E14NS, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chlorophyll
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metabolism Fluorescence Recovery After Photobleaching Freeze Fracturing Intracellular Membranes / metabolism*, ultrastructure Kinetics Light-Harvesting Protein Complexes / metabolism* Models, Molecular Photochemical Processes* Photosystem II Protein Complex / metabolism* Plant Proteins / metabolism Protein Transport Spinacia oleracea / metabolism*, ultrastructure Thermodynamics Thylakoids / metabolism*, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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//Biotechnology and Biological Sciences Research Council; //Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Light-Harvesting Protein Complexes; 0/Photosystem II Protein Complex; 0/Plant Proteins; 1406-65-1/Chlorophyll |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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