Document Detail


Photoproducts formed from photofrin II in cells.
MedLine Citation:
PMID:  2977184     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fluorescence and absorption spectra of light-exposed cells containing the tumour-localizing porphyrin preparation Photofrin II (PII) have been studied. Light exposure results in spectral changes that may be due to a photoinduced modification of the porphyrins without breakage of the porphyrin macrocycle and/or to a photoinduced displacement of the porphyrins in the cells. Photochemical reaction involving breakage of the porphyrin macrocycle also occur as can be seen from the loss of absorbance within the Soret band region during light exposure. Singlet oxygen may be involved in the photodegradation of PII in cells since the process is slowed down on bubbling N2 through the samples and is slightly faster in suspensions in Dulbecco's phosphate buffered saline (PBS) made of D2O compared with suspensions in PBS made of H2O. During light exposure a fluorescent product is formed in the cells with fluorescence excitation and emission characteristics similar to those of the "age pigment" lipofuscin (lambda exc = 350 nm, lambda em = 440 nm).
Authors:
J Moan; D Kessel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  1     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  1988 May 
Date Detail:
Created Date:  1989-05-19     Completed Date:  1989-05-19     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  429-36     Citation Subset:  IM    
Affiliation:
Institute for Cancer Research, Montebello, Oslo, Norway.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Female
Hematoporphyrin Derivative
Hematoporphyrins / metabolism*
Humans
Kinetics
Light
Radiation-Sensitizing Agents / metabolism*
Spectrometry, Fluorescence
Tumor Cells, Cultured / metabolism*,  radiation effects
Ultraviolet Rays
Uterine Cervical Neoplasms
Chemical
Reg. No./Substance:
0/Hematoporphyrins; 0/Radiation-Sensitizing Agents; 68335-15-9/Hematoporphyrin Derivative

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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