| Photophysics and photochemistry of the UV filter kynurenine covalently attached to amino acids and to a model protein. | |
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MedLine Citation:
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PMID: 20722363 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The photophysics and photochemistry of kynurenine (KN) covalently bound to the amino acids lysine, cysteine, and histidine, the antioxidant glutathione, and the protein lysozyme have been studied by optical spectroscopy with femto- and nanosecond time resolution. The fluorescence quantum yield of the adducts of KN to amino acids is approximately 2 times higher than that of the free KN in solution; KN attached to protein exhibits a 7-fold increase in the fluorescence quantum yield. The S(1) state dynamics of KN-modified lysozyme reveals a multiphasic decay with a broad dispersion of time constants from 1 ps to 2 ns. An increase of the triplet yield of KN bound to lysozyme is also observed; the triplet state undergoes fast intramolecular decay. The obtained results reveal an increase of the photochemical activity of KN after its covalent attachment to amino acids and proteins, which may contribute to the development of oxidative stress in the human lenses-the main causative factor for the cataract onset. |
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Authors:
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Peter S Sherin; Jakob Grilj; Lyudmila V Kopylova; Vadim V Yanshole; Yuri P Tsentalovich; Eric Vauthey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The journal of physical chemistry. B Volume: 114 ISSN: 1520-5207 ISO Abbreviation: J Phys Chem B Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2011-01-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101157530 Medline TA: J Phys Chem B Country: United States |
Other Details:
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Languages: eng Pagination: 11909-19 Citation Subset: IM |
Affiliation:
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International Tomography Center SB RAS, Institutskaya str. 3a, 630090 Novosibirsk, Russia. Sherin@tomo.nsc.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry* Animals Chickens Cysteine / chemistry Glutathione / chemistry Histidine / chemistry Humans Kynurenine / chemistry* Lasers Light Lysine / chemistry Molecular Structure Muramidase / chemistry, genetics Peptides / chemistry, genetics Photochemistry / methods* Spectrometry, Fluorescence / methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Ultraviolet Rays* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Peptides; 343-65-7/Kynurenine; 52-90-4/Cysteine; 56-87-1/Lysine; 70-18-8/Glutathione; 71-00-1/Histidine; EC 3.2.1.-/hen egg lysozyme; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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