Document Detail

Photoperiodic expression of two RALDH enzymes and the regulation of cell proliferation by retinoic acid in the rat hypothalamus.
MedLine Citation:
PMID:  22681644     Owner:  NLM     Status:  MEDLINE    
Retinoic acid (RA) has been found to regulate hypothalamic function, but precisely where it acts is unknown. This study shows expression of retinaldehyde dehydrogenase (RALDH) enzymes in tanycytes that line the third ventricle in an area overlapping with the site of hypothalamic neural stem cells. The influence of RA was examined on the proliferation of progenitors lining the third ventricle using organotypic slice cultures. As has been shown in other regions of neurogenesis, RA was found to inhibit proliferation. Investigations of the dynamics of RALDH1 expression in the rat hypothalamus have shown that this enzyme is in tanycytes under photoperiodic control with highest levels during long versus short days. In parallel to this shift in RA synthesis, cell proliferation in the third ventricle was found to be lowest during long days when RA was highest, implying that RALDH1 synthesized RA may regulate neural stem cell proliferation. A second RA synthesizing enzyme, RALDH2 was also present in tanycytes lining the third ventricle. In contrast to RALDH1, RALDH2 showed little change with photoperiodicity, but surprisingly the protein was present in the apparent absence of mRNA transcript and it is hypothesized that the endocytic tanycytes may take this enzyme up from the cerebrospinal fluid (CSF).
Kirsty D Shearer; Patrick N Stoney; Sonia E Nanescu; Gisela Helfer; Perry Barrett; Alexander W Ross; Peter J Morgan; Peter McCaffery
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-07-09
Journal Detail:
Title:  Journal of neurochemistry     Volume:  122     ISSN:  1471-4159     ISO Abbreviation:  J. Neurochem.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-18     Completed Date:  2012-09-27     Revised Date:  2014-10-14    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  789-99     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Journal of Neurochemistry © 2012 International Society for Neurochemistry.
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MeSH Terms
Blotting, Western
Cell Proliferation / drug effects*
Cells, Cultured
Gene Expression Regulation, Enzymologic / drug effects
Hypothalamus / cytology*,  drug effects,  enzymology*
In Situ Hybridization
Isoenzymes / biosynthesis
Mice, Inbred C57BL
Neural Stem Cells / drug effects
Organ Culture Techniques
Polymerase Chain Reaction
Rats, Inbred F344
Rats, Sprague-Dawley
Retinal Dehydrogenase / biosynthesis*,  cerebrospinal fluid
Third Ventricle / cytology,  drug effects,  metabolism
Tretinoin / analysis,  pharmacology*
Grant Support
BB/G014272/1//Biotechnology and Biological Sciences Research Council; //Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Isoenzymes; 5688UTC01R/Tretinoin; EC protein, rat; EC protein, rat; EC Dehydrogenase

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