| Photokilling mechanisms induced by zinc(II)-phthalocyanine on cultured tumor cells. | |
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MedLine Citation:
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PMID: 10850885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The photosensitizing effects of liposomal zinc(II)-phthalocyanine (ZnPc) on HeLa cells, with emphasis on morphological changes and mechanisms for cell death, have been studied. No dark toxicity for ZnPc alone was found. Incubation for 1 h with ZnPc followed by red light irradiation induced a variable decrease in the surviving of cells, which was related to both drug concentration and irradiation time. A lethal photodynamic effect (100% of the cells are killed: LD100) was induced by 5 x 10-6 M ZnPc and 5-min irradiation, whereas a sublethal effect (60% of the cells are killed: LD60) was detected with 10 7 M ZnPc and 3 min of red light. Toluidine blue and Hoechst 33258 staining showed characteristic alterations of cell morphology. Numerous bubbles on the plasma membrane were found immediately after an LD100 treatment, and a necrotic morphology appeared 24 h later. On the contrary, severe cell shrinkage with nuclear fragmentation. characteristic of apoptosis. was observed 8 and 24 h after LD60 treatments. In this case, propidium iodide-acridine orange labeling and the TUNEL assay confirmed the occurrence of apoptosis. The highest amount of apoptotic cells appeared 24 h after LD60 treatments, particularly in detached cells, as revealed by cell counting and DNA electrophoresis. Both apoptotic and necrotic mechanisms for cell death occur in HeLa cells in dependence on the experimental protocol of ZnPc photodynamic treatments. |
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Authors:
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A Villanueva; V Domínguez; S Polo; V D Vendrell; C Sanz; T M Cañete; A Juarranz; J C Stockert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncology research Volume: 11 ISSN: 0965-0407 ISO Abbreviation: Oncol. Res. Publication Date: 1999 |
Date Detail:
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Created Date: 2000-07-13 Completed Date: 2000-07-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9208097 Medline TA: Oncol Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 447-53 Citation Subset: IM |
Affiliation:
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Departamento de Biologiá, Facultad de Ciencias, Universidad Autónoma de Madrid, Canto Blanco, Spain. angeles.villanueva@uam.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis Cell Survival / drug effects, radiation effects Dose-Response Relationship, Drug Electrophoresis, Agar Gel Hela Cells Humans Indoles / pharmacology* Kinetics Light Liposomes Microscopy, Fluorescence Necrosis Organometallic Compounds / pharmacology* Photochemotherapy / methods* Photosensitizing Agents / pharmacology* Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/Liposomes; 0/Organometallic Compounds; 0/Photosensitizing Agents; 14320-04-8/Zn(II)-phthalocyanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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