Document Detail

Photoisomerization of retinoic acid and its influence on regulation of human keratinocyte growth and differentiation.
MedLine Citation:
PMID:  10983416     Owner:  NLM     Status:  MEDLINE    
Retinoic acid constantly undergoes structural inter-conversions among the geometrical isomers (all-trans-retinoic acid, 9-cis-retinoic acid, 11-cis-retinoic acid, 13-cis-retinoic acid and 9-13-di-cis-retinoic acid) by photoisomerization under natural light. Geometric isomers of retinoic acid thus formed showed different effects on human epidermal keratinocyte growth and differentiation. The ability of the isomers to inhibit the synthesis of cornified envelope (terminal event in the keratinocyte differentiation program) changed rapidly when illuminated by white fluorescent light. The 11-cis-retinoic acid had a 3-fold stronger activity to inhibit the growth of keratinocytes than the other geometric isomers. On the other hand, all-trans-retinoic acid, 9-cis-retinoic acid and 9-13-di-cis-retinoic acid exhibited a 3-fold greater ability to inhibit synthesis of involucrin, transglutaminase and the cornified envelopes. The regulation of keratin expression by the geometric isomers of retinoic acids was extremely complex. Level of keratin-1 (K1) mRNA was increased by 11-cis-retinoic acid and 13-cis-retinoic acid, but suppressed by 9,13-di-cis-retinoic acids while all-trans-retinoic acid and 9-cis-retinoic acid had no effect. Level of keratin-10 (K10) mRNA was strongly inhibited by all-trans-retinoic acid, 9-cis-retinoic acid and 11-cis-retinoic acid as compared to 13-cis-retinoic acid and 9,13-di-cis-retinoic acids. The mRNA level of keratin-14 (K14) was suppressed by all-trans-retinoic acid, 9-cis-retinoic acid and 11-cis-retinoic acid but not influenced by 13-cis-retinoic acid and 9,13-di-cis-retinoic acid. Natural light induced structural inter-conversions among the geometric isomers of retinoic acids in tissues-especially the skin, might play a crucial role in the regulation of growth and differentiation of keratinocytes.
S R Kunchala; T Suzuki; A Murayama
Related Documents :
8538206 - Butyrate and phenylacetate as differentiating agents: practical problems and opportunit...
17710236 - Role of ppars and retinoid x receptors in the regulation of lung maturation and develop...
11600826 - Hiv-protease inhibitors alter retinoic acid synthesis.
3578676 - Ethacrynic acid effects on the isolated inner ear: evaluation of the ototoxic potential...
9023016 - Activation of a cyclic nucleotide phosphodiesterase 4 (pde4) from rat thymocytes by pho...
10420586 - Ganglioside gd1 alpha analogues as high-affinity ligands for myelin-associated glycopro...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of biochemistry & biophysics     Volume:  37     ISSN:  0301-1208     ISO Abbreviation:  Indian J. Biochem. Biophys.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0310774     Medline TA:  Indian J Biochem Biophys     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  71-6     Citation Subset:  IM    
Centre for Cellular and Molecular Biology, Hyderabad, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Differentiation / drug effects
Cell Division / drug effects
Cells, Cultured
Keratinocytes / cytology,  drug effects*,  metabolism
Keratins / genetics
RNA, Messenger / genetics,  metabolism
Tretinoin / chemistry*,  pharmacology*,  radiation effects
Reg. No./Substance:
0/RNA, Messenger; 302-79-4/Tretinoin; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Urate oxidase electrode based on dissolved oxygen probe for urine uric acid determination.
Next Document:  Glutaraldehyde cross-linking of lectins to marker enzymes: protection of binding site by specific su...