Document Detail


Photoinactivation of Candida albicans by its own endogenous porphyrins.
MedLine Citation:
PMID:  19795168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The possibility of photoeradicating the prokaryotic microorganism Candida albicans by enhancing its endogenous porphyrin production and accumulation was investigated in this study. Induction of porphyrin synthesis was performed by the addition of delta-aminolevulinic acid (ALA), or its hydrophobic derivative ALA methyl ester (m-ALA). Photoinactivation of C. albicans was performed under blue light (407-420 nm) illumination. A decrease in viability of about 1.6 or 2.1 orders of magnitudes was obtained with a light dose of 36 J/cm(2) for an initial concentration of 100-mg/ml ALA or m-ALA, respectively. Endogenous porphyrins extracted from the cells showed that cultures incubated with m-ALA accumulated a relatively higher amount of endogenous porphyrins than ALA, indicating better transport through the yeast cell barriers. When a combination of miconazole and ketoconazole (antifungal agents) is given at a sub-inhibitory concentration (0.5 microg/ml each) with an inducer, a 2.1 or 3.2 orders of magnitude decrease in viability is caused with ALA or with m-ALA, respectively, upon illumination. Fluorescence intensities of the accumulated porphyrins as demonstrated by FACS indicate that the combination of the two azole drugs and an inducer cause a relatively high amount of endogenous porphyrins. Although the additive action of both azole drugs allow better penetration of the inducer, especially m-ALA photoeradication remained limited because of an acidic pH generated in the presence of the inducer. The acidic pH is probably the cause for the inefficiency of the photodynamic treatment. More hydrophobic inducers than m-ALA and less acidic must be investigated to improve the photodynamic treatment by endogenous-induced porphyrins.
Authors:
Sarit Oriel; Yeshayahu Nitzan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-01
Journal Detail:
Title:  Current microbiology     Volume:  60     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-21     Completed Date:  2010-02-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  117-23     Citation Subset:  IM    
Affiliation:
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. sarit_o2003@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Aminolevulinic Acid / analogs & derivatives,  metabolism
Antifungal Agents / pharmacology
Azoles / pharmacology
Candida albicans / drug effects,  metabolism,  radiation effects*
Light
Microbial Viability / drug effects,  radiation effects*
Porphyrins / biosynthesis*
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Azoles; 0/Porphyrins; 106-60-5/Aminolevulinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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