Document Detail


Photodynamic treatment of yeast cells with the dye toluidine blue: all-or-none loss of plasma membrane barrier properties.
MedLine Citation:
PMID:  1322699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic treatment of Kluyveromyces marxianus with the sensitizer Toluidine blue leads to the loss of colony forming capacity. In this paper, the influence of this treatment on the barrier properties of the plasma membrane has been studied. Photodynamic treatment with the dye Toluidine blue resulted in efflux of potassium ions and E260-absorbing material. Moreover, cells became stainable with erythrosine. It is concluded that the permeability change induced by photodynamic treatment proceeds in an all-or-none fashion. Treatment of this yeast strain, with the dye and light, also induced a diminution of the cell volume. This process is most likely not coupled to the cellular potassium content, but rather to the integrity of the vacuole. These data suggest that the vacuole has an important function in the maintenance of cell volume. Finally, it was observed that the loss of cell viability was not induced by the all-or-none loss of barrier properties.
Authors:
M Paardekooper; P J Van den Broek; A W De Bruijne; J G Elferink; T M Dubbelman; J Van Steveninck
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1108     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-09-08     Completed Date:  1992-09-08     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  86-90     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, State University Leiden, Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Arginine / metabolism
Cell Membrane Permeability / drug effects*
Erythrosine / pharmacology
Kinetics
Kluyveromyces / cytology,  drug effects*,  growth & development
Light
Photochemistry
Potassium / metabolism
Tolonium Chloride / pharmacology*
Chemical
Reg. No./Substance:
16423-68-0/Erythrosine; 74-79-3/Arginine; 7440-09-7/Potassium; 92-31-9/Tolonium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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