Document Detail


Photodynamic therapy with local photosensitizer delivery inhibits experimental intimal hyperplasia.
MedLine Citation:
PMID:  9888322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Photodynamic therapy (PDT), the light activation of photosensitizer dyes for the production of free radicals, effectively inhibits experimental intimal hyperplasia with systemic administration of the photosensitizer. The local application of the photosensitizer directly into a vascular lesion to avoid systemic side effects and tightly control dose administration has theoretical appeal. The aim of this study was to quantify serum and arterial tissue uptake after site-specific photosensitizer delivery and, following PDT, determine its effectiveness at inhibiting intimal hyperplasia. STUDY DESIGN/MATERIALS AND METHODS: The rat common carotid artery was balloon-injured, pressurized at 400 mm Hg for 2 minutes with the photosensitizer dye benzoporphyrin-derivative (BPD), and irradiated with 690 nm laser light at a fluence of 100 J/cm2. Control animals were pressurized with saline only, or received no additional treatment than balloon-injury. RESULTS: Pressurization with BPD resulted in complete penetration of the intima and media and was associated with relatively high tissue, but almost no detectable serum BPD concentrations. No skin photosensitization or other systemic side effects were observed with photosensitizer administration. After 9 days, PDT-treated arteries displayed a significantly lower number of smooth muscle cells in the arterial wall than balloon-injured (P < 0.001) or saline-pressurized arteries (P < 0.0002), and no intimal hyperplasia. At 21 days, IH after PDT was significantly reduced as compared with balloon-injured (P < 0.0004), or saline-pressurized arteries (P < 0.003) with no arterial dilatation. CONCLUSIONS: Site-specific delivery of liposomal BPD followed by PDT represents a safe method to treat arteries, and may be effectively used in vivo to inhibit the development of intimal hyperplasia.
Authors:
F Adili; R G Statius van Eps; T J Flotte; G M LaMuraglia
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  23     ISSN:  0196-8092     ISO Abbreviation:  Lasers Surg Med     Publication Date:  1998  
Date Detail:
Created Date:  1999-03-22     Completed Date:  1999-03-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  263-73     Citation Subset:  IM    
Affiliation:
Division of Vascular Surgery, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / drug effects*,  metabolism,  pathology*
Drug Evaluation, Preclinical
Hyperplasia / drug therapy,  metabolism,  pathology
Immunohistochemistry
Injections, Intralesional / methods
Laser Therapy
Male
Photochemotherapy / methods*
Photosensitizing Agents / administration & dosage*,  pharmacokinetics
Porphyrins / administration & dosage*,  pharmacokinetics
Random Allocation
Rats
Rats, Sprague-Dawley
Tunica Intima / drug effects*,  metabolism,  pathology*
Grant Support
ID/Acronym/Agency:
HL02583/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Photosensitizing Agents; 0/Porphyrins; 129497-78-5/verteporfin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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