Document Detail


Photodynamic therapy with hexenyl ester of 5-aminolevulinic acid induces necrotic cell death in salivary gland adenocarcinoma cells.
MedLine Citation:
PMID:  20514459     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy has been developed as an alternative therapy of cancer. The aim of this study was to examine whether PDT with hexenyl ester of 5-aminolaevulinic acid (ALA-hx) inhibits the proliferation of the salivary gland adenocarcinoma SGT cells. Cell proliferation was examined by MTT assay. The gene expression of Coproporphyrinogen oxidase (CPO) and ROS production was also examined. Flow cytometry and in vivo Chorioallantoic membrane (CAM) assay was performed. ALA-hx PDT inhibited effectively the proliferation of SGT cells. Treatment of ALA-hx induced CPO mRNA expression and ROS was produced by ALA-hx PDT in SGT cells. Flow cytometry and LDH assay showed that ALA-hx PDT induced necrotic cell death rather than apoptosis in SGT cells. In vivo CAM assay showed that ALA-hx PDT induced tumor destruction by inducing necrosis. These results indicate that ALA-hx PDT effectively inhibits the proliferation of SGT cells by inducing necrosis.
Authors:
Jong-Hwan Park; Yeon-Hee Moon; Dong-Jae Kim; Soo-A Kim; Jee-Bum Lee; Sang-Gun Ahn; Jung-Hoon Yoon
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  24     ISSN:  1791-2431     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-01     Completed Date:  2010-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  177-81     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, College of Medicine, Konyang University, Daejon 302-718, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / drug therapy*,  genetics,  metabolism,  pathology*
Aminolevulinic Acid / analogs & derivatives,  pharmacology,  therapeutic use
Animals
Antineoplastic Agents / pharmacology,  therapeutic use
Cell Death / drug effects,  genetics
Cell Line, Tumor
Cell Survival / drug effects,  genetics
Chick Embryo
Coproporphyrinogen Oxidase / genetics,  metabolism
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Humans
Necrosis / chemically induced,  genetics,  metabolism
Neoplasm Invasiveness
Photochemotherapy* / methods
Reactive Oxygen Species / metabolism
Salivary Gland Neoplasms / drug therapy*,  genetics,  metabolism,  pathology*
Chemical
Reg. No./Substance:
0/5-aminolevulinic acid hexyl ester; 0/Antineoplastic Agents; 0/Reactive Oxygen Species; 106-60-5/Aminolevulinic Acid; EC 1.3.3.3/Coproporphyrinogen Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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