| Photodynamic therapy for pythiosis. | |
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MedLine Citation:
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PMID: 23331689 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background - Pythiosis is a life-threatening disease caused by Pythium insidiosum. Photodynamic therapy (PDT) is an alternative treatment to surgery that uses the interaction of a photosensitizer, light and molecular oxygen to cause cell death. Objectives - To evaluate the effect of PDT on the in vitro growth of P. insidiosum and in an in vivo model of pythiosis. Methods - For in vitro studies, two photosensitizers were evaluated: a haematoporphyrin derivative (Photogem(®) ) and a chlorine (Photodithazine(®) ). Amphotericin B was also evaluated, and the control group was treated with sterile saline solution. All experiments (PDT, porphyrin, chlorine and light alone, amphotericin B and saline solution) were performed as five replicates. For in vivo studies, six rabbits were inoculated with 20,000 zoospores of P. insidiosum, and an area of 1 cm(3) was treated using the same sensitizers. The PDT irradiation was performed using a laser emitting at 660 nm and a fluence of 200 J/cm(2) . Rabbits were clinically evaluated daily and histopathological analysis was performed 72 h after PDT. Results - For in vitro assays, inhibition rates for PDT ranged from 60 to 100% and showed better results in comparison to amphotericin B. For the in vivo assays, after PDT, histological analysis of lesions showed a lack of infection up to 1 cm in depth. Conclusions and clinical importance - In vitro and in vivo studies showed that PDT was effective in the inactivation of P. insidiosum and may represent a new approach to treating pythiosis. |
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Authors:
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Layla Pires; Sandra de M G Bosco; Nelson F da Silva Junior; Cristina Kurachi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Veterinary dermatology Volume: 24 ISSN: 1365-3164 ISO Abbreviation: Vet. Dermatol. Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-01-21 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9426187 Medline TA: Vet Dermatol Country: England |
Other Details:
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Languages: eng Pagination: 130-e30 Citation Subset: IM |
Copyright Information:
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© 2013 The Authors. Veterinary Dermatology © 2013 ESVD and ACVD. |
Affiliation:
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Physics Institute of São Carlos and Instituto de Biociências de Botucatu, Univ Estadual Paulista, Campus Botucatu, Departamento de Microbiologia e Imunologia, Distrito de Rubião Júnior, S/N, 18618-970, Botucatu-SP, Brazil Escola de Engenharia de São Carlos, University of São Paulo, Avenida Trabalhador São-Carlense, 400, Parque Arnold Schimidt, 13566-590, São Carlos-SP, Brazil. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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