Document Detail


Photodynamic therapy disinfection of carious tissue mediated by aluminum-chloride-phthalocyanine entrapped in cationic liposomes: an in vitro and clinical study.
MedLine Citation:
PMID:  21809069     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Photodynamic therapy (PDT) is a technique employed in the treatment of several superficial infections, such as caries. PDT uses a non-toxic drug termed photosensitizer (PS) followed by light irradiation. The cytotoxic effects of the therapy are related to the production of reactive species produced after light activation of a photosensitizer, which reacts with surrounding molecules and disrupts several of the cell's functions. Within this context, this study aimed to develop a clinical protocol involving PDT application mediated by aluminum-chloride-phthalocyanine (AlClPc) entrapped in cationic liposomes against cariogenic bacteria in caries lesions. Cationic liposomes were used to delivery AlClPc preferentially to bacterial cells due to the strong anionic superficial charges of these cell types. The results are represented in two fundamental steps: (1) in vitro evaluation of AlClPc delivery to cariogenic bacteria and pulp cells, as well as its potential phototoxicity; (2) a clinical study involving volunteer patients that were treated with the PDT protocol mediated by AlClPc-cationic liposome. The main results showed that the AlClPc-cationic liposome was preferentially absorbed by bacterial cells compared to eukaryotic dental pulp cells, and it was efficient in the reduction of microbial load from bacterial cultures. In addition, the clinical study showed a mean reduction of 82% of total bacterial in the treated cavities after PDT application. Taken together, the results presented in this study showed that the antimicrobial PDT protocol mediated by cationic liposomes containing AlClPc is safety for clinical application and is efficient in the reduction of bacterial load in caries lesions.
Authors:
João Paulo F Longo; Soraya C Leal; Andreza R Simioni; Maria de Fátima Menezes Almeida-Santos; Antônio C Tedesco; Ricardo B Azevedo
Related Documents :
7229929 - Comparison of quantitation methods for l-1210 cell populations and evaluation of select...
19733199 - Discrimination of viable and dead vibrio vulnificus after refrigerated and frozen stora...
19768799 - Ultra scale-down approaches for clarification of mammalian cell culture broths in disc-...
2329479 - Study of the lens epithelial cell density in cataractous eyes operated on with extracap...
17947119 - Image analysis for neuroblastoma classification: segmentation of cell nuclei.
21284649 - Interpreting tree responses to thinning and fertilization using tree-ring stable isotopes.
19628339 - Sirna targeting survivin inhibits growth and induces apoptosis in human renal clear cel...
21769329 - Targeted ph-dependent fluorescent activity-based cathepsin probes.
22874769 - Colon cancer cell chemosensitisation by fish oil emulsion involves apoptotic mitochondr...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-2
Journal Detail:
Title:  Lasers in medical science     Volume:  -     ISSN:  1435-604X     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-2     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8611515     Medline TA:  Lasers Med Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Genetics and Morphology, Institute of Biology, University of Brasília, Brasilia, DF, CEP-70910-900, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Bisphosphonate-related osteonecrosis: laser-assisted surgical treatment or conventional surgery?
Next Document:  The effect of an Er,Cr:YSGG laser on the micro-shear bond strength of composite to the enamel and de...