Document Detail

Photodynamic therapy associating Photogem and blue LED on L929 and MDPC-23 cell culture.
MedLine Citation:
PMID:  19947913     Owner:  NLM     Status:  MEDLINE    
To evaluate the cytotoxicity of PDT (photodynamic therapy) with Photogem associated to blue LED (light-emitting diode) on L929 and MDPC-23 cell cultures, 30000 cells/cm2 were seeded in 24-well plates for 48 h, incubated with Photogem (10, 25 or 50 mg/l) and irradiated with an LED source (460+/-3 nm; 22 mW/cm2) at two energy densities (25.5 or 37.5 J/cm2). Cell metabolism was evaluated by the MTT (methyltetrazolium) assay (Dunnet's post hoc tests) and cell morphology by SEM (scanning electron microscopy). Flow cytometry analysed the type of PDT-induced cell death as well and estimated intracellular production of ROS (reactive oxygen species). There was a statistically significant decrease of mitochondrial activity (90% to 97%) for all Photogem concentrations associated to blue LED, regardless of irradiation time. It was also demonstrated that the mitochondrial activity was not recovered after 12 or 24 h, characterizing irreversible cell damage. PDT-treated cells presented an altered morphology with ill-defined limits. In both cell lines, there was a predominance of necrotic cell death and the presence of Photogem or irradiation increased the intracellular levels of ROS. PDT caused severe toxic effects in normal cell culture, characterized by the reduction of the mitochondrial activity, morphological alterations and induction of necrotic cell death.
Ana Paula Dias Ribeiro; Ana Cláudia Pavarina; Flávia Zardo Trindade; Natália Mayumi Inada; Vanderlei Salvador Bagnato; Carlos Alberto de Souza Costa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-08
Journal Detail:
Title:  Cell biology international     Volume:  34     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-06-24     Revised Date:  2010-11-12    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  343-51     Citation Subset:  IM    
Department of Dental Materials and Prosthodontics, São Paulo State University, Araraquara School of Dentistry, Araraquara, SP, Brazil.
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MeSH Terms
Cell Line
Flow Cytometry
Microscopy, Electron, Scanning
Mitochondria / drug effects,  radiation effects
Photochemotherapy / adverse effects*
Photosensitizing Agents / toxicity*
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Photosensitizing Agents; 0/Reactive Oxygen Species

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