Document Detail


Photodynamic sensitization of Leishmania amazonensis in both extracellular and intracellular stages with aluminum phthalocyanine chloride for photolysis in vitro.
MedLine Citation:
PMID:  16251285     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Leishmania amazonensis, a causative agent of cutaneous leishmaniasis, is susceptible in vitro to light-mediated cytolysis in the presence of or after pretreatment with the photosensitizer aluminum phthalocyanine chloride. Cytolysis of both promastigotes and axenic amastigotes required less photosensitizer (e.g., one microg.ml(-1)) and a lower light dose (e.g., 1.5 J.cm(-2)) than did the mammalian cells examined for comparison. Exposure of Leishmania cells to the photosensitizer alone had little effect on their viability, as judged from their motility, growth, and/or retention of green fluorescent proteins genetically engineered for episomal expression. Fluorimetric assays for cell-associated and released green fluorescence proteins proved to be even more sensitive for the evaluation of cell viability than microscopy for the evaluation of motility and/or integrity. Axenic amastigotes pretreated with the photosensitizer infected macrophages of the J774 line but were lysed intracellularly when the infected cells were exposed to light. Addition of the photosensitizer to the already infected cells produced no effect on their intracellular parasites. However, light irradiation lysed these macrophages and also those infected with parasites preincubated with the photosensitizer at a concentration of 5 microg.ml(-1) or higher. Photosensitized Leishmania cells are highly susceptible to cytolysis, apparently due to the generation of reactive oxidative species on light illumination, suggestive of inefficiency of their antioxidant mechanisms. Efficient delivery of photosensitizers to intracellular Leishmania is expected to increase their therapeutic potentials against leishmaniasis.
Authors:
Sujoy Dutta; Debalina Ray; Bala K Kolli; Kwang-Poo Chang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  49     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-27     Completed Date:  2006-01-18     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4474-84     Citation Subset:  IM    
Affiliation:
Department of Microbiology/Immunology, Chicago Medical School, Rosalind Franklin University, 3333 Green Bay Rd., N. Chicago, Illinois 60064, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Indoles / pharmacology*
Leishmania mexicana / drug effects*
Mice
Organometallic Compounds / pharmacology*
Photochemotherapy*
Photolysis*
Photosensitizing Agents / pharmacology*
Grant Support
ID/Acronym/Agency:
AI-20486/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Indoles; 0/Organometallic Compounds; 0/Photosensitizing Agents; 14154-42-8/chloroaluminum phthalocyanine
Comments/Corrections

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