Document Detail

Photodynamic properties of ZnTPPS(4), ClAlPcS(2) and ALA in human melanoma G361 cells.
MedLine Citation:
PMID:  19720133     Owner:  NLM     Status:  MEDLINE    
Photodynamic therapy (PDT) has been approved as proper and effective kind of treatment for certain types of cancer and non-malignant diseases. We tested photodynamic effects on G361 human melanoma cells sensitized by zinc-5,10,15,20-tetrakis(4-sulphonatophenyl) porphyrine (ZnTPPS(4)), chloraluminium phtalocyanine disulfonate (ClAlPcS(2)) and 5-aminolevulinic acid (ALA). In particular, we examined the PDT efficiency depending on applied light dose (0.8; 1.7; 3.3; 6.6; 13.2; 26.4Jcm(-2)). The DNA gel electrophoresis, methylthiazol tetrazolium bromide (MTT) viability test, fluorescent microscopy using calcein AM and propidium iodide (PI) staining, and rhodamine 123 mitochondrial membrane potential assay were performed to detect and evaluate the cell death process. We also measured the time course of reactive oxygen species (ROS) production and its dependence on sensitizer concentration within continuously irradiated sensitized cells. In conclusion, these results demonstrate most significant phototoxic effect of ClAlPcS(2)-PDT in spite of significantly higher ROS production induced by ZnTPPS(4)-PDT on G361 cells. On the other hand, ALA-PDT has a minimal photoeffect and induces negligible ROS formation in G361 cells at the conditions described below.
E Krestyn; H Kolarova; R Bajgar; K Tomankova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-29
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  24     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  286-91     Citation Subset:  IM    
Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Olomouc, Czech Republic.
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MeSH Terms
Aminolevulinic Acid / pharmacology*
Cell Line, Tumor
Coloring Agents
DNA Fragmentation / drug effects,  radiation effects
Dose-Response Relationship, Radiation
Electrophoresis, Polyacrylamide Gel
Fluorescent Dyes
Indoles / pharmacology*
Melanoma, Experimental / therapy*
Membrane Potentials / drug effects
Metalloporphyrins / pharmacology*
Microscopy, Fluorescence
Mitochondrial Membranes / drug effects
Organometallic Compounds / pharmacology*
Photosensitizing Agents / pharmacology*
Reactive Oxygen Species / metabolism
Rhodamine 123
Tetrazolium Salts
Reg. No./Substance:
0/Coloring Agents; 0/Fluorescent Dyes; 0/Indoles; 0/Metalloporphyrins; 0/Organometallic Compounds; 0/Photosensitizing Agents; 0/Reactive Oxygen Species; 0/Tetrazolium Salts; 0/Thiazoles; 0/zinc-tetrakis(p-sulfonatophenyl)porphyrin; 106-60-5/Aminolevulinic Acid; 298-93-1/thiazolyl blue; 36015-30-2/Propidium; 62669-70-9/Rhodamine 123; 68637-19-4/aluminum phthalocyanine disulfonate

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