| Photodynamic inhibition of acetylcholinesterase after two-photon excitation of copper tetrasulfophthalocyanine. | |
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MedLine Citation:
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PMID: 17384975 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sequential two-photon (2-gamma) activated copper tetrasulfophthalocyanine (CuPcS(4)) was shown capable of inactivating acetylcholinesterase (ACE). ACE activity was measured photometrically by the Ellman method. Simultaneous irradiation of ACE in the presence of CuPcS(4) with 514 nm (183 mW/cm(2)) and 670 nm (86 mW/cm(2)) continuous wave (CW) light induced a 20-50% increase in enzyme inhibition as compared to one-photon (1-gamma) irradiation, using either 514- or 670-nm (CW) light at the same fluences. The enzyme activity was not affected by CuPcS(4) or light alone, decreased linearly with the irradiation time, and was shown to be oxygen-dependent. We conclude that the photoinactivation of ACE with sequential 2-gamma irradiation involves reactive oxygen species produced by the interaction of the upper excited T(n) state of CuPcS(4) with molecular oxygen. As CuPcS(4) shows little activity as a conventional 1-gamma photosensitizer, unwanted side effects such as prolonged skin sensitivity are eliminated rendering 2-gamma photodynamic therapy advantageous for the treatment of selected medical applications. |
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Authors:
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Youssef Mir; Daniel Houde; Johan E van Lier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-03-24 |
Journal Detail:
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Title: Lasers in medical science Volume: 23 ISSN: 0268-8921 ISO Abbreviation: Lasers Med Sci Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2007-11-28 Completed Date: 2008-03-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8611515 Medline TA: Lasers Med Sci Country: England |
Other Details:
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Languages: eng Pagination: 19-25 Citation Subset: IM |
Affiliation:
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Department of Nuclear Medicine and Radiobiology,Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, J1H 5N4, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholinesterase
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drug effects* Indoles / chemistry*, toxicity Photochemotherapy / methods* Photons Photosensitizing Agents / chemistry*, toxicity Reactive Oxygen Species |
| Chemical | |
Reg. No./Substance:
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0/Indoles; 0/Photosensitizing Agents; 0/Reactive Oxygen Species; 33308-41-7/tetrasulfophthalocyanine; EC 3.1.1.7/Acetylcholinesterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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